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Antibodies

Rabbit IgG Fc Antibody DyLight[TM] 680 Conjugated

Product Sizes
100 ug
611-144-003-100UG
About this Product
SKU:
611-144-003
Additional Names:
Goat Anti Rabbit IgG F(c) DyLight 680™ Conjugated Antibody, Goat Anti-Rabbit IgG Fc Fragment Antibody DyLight 680™ conjugation, Goat Anti Rabbit IgG Fc Antibody DyLight 680™ conjugated
Application:
FLISA, Immunofluorescence, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
DyLight™ 680
Concentration:
1 mg/ml
Extra Details:
Anti-Rabbit IgG F(c) DyLight generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of rabbit IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Rabbit IgG F(c) fragment
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Rabbit
Shipping Conditions:
Ambient
Specificity:
IgG Fc|This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against Rabbit IgG F(ab).
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
Immunoprecipitation and western blot show interaction of otoferlin with CaMKIID Delta. (A-C) Two HA-tagged mouse otoferlin fragments, C2ABC (aa 1-632 in NP_001093865; 70 kDa) and C2DEF (aa 933-1920; 114 kDa) were co-transfected with mcherry-tagged mouse CaMKIID Delta into HEK293 cells. Transfections were performed either with otoferlin C2ABC and CaMKIID Delta (A, Input Lane 1 and 2), otoferlin C2DEF and CaMKIID Delta (B, Input Lane 1 and 2) or in the presence of both C2ABC and C2DEF fragments and CaMKIID Delta (C, Input Lane 1 and 2). Co-immunoprecipitations of C2ABC-HA and C2DEF-HA were conducted from HEK293 cell lysates using anti-HA antibodies (p/n 600-401-384). CaMKIID Delta-mcherry was detected in the eluate using an anti-RFP (red fluorescent protein) antibody (p/n 200-301-379) (A-C, Lane 3), indicating that CaMKIID Delta co-precipitated with recombinant otoferlin fragments. Secondary anti-rabbit Dylight680 (p/n 611-144-003) and anti-mouse Dylight800 antibodies (610-145-003) (1:10,000). FIGURE 5. PMID: 29046633.
C. Representative western blots, original blots are shown in (supplementary Fig S8-9). And densitometric quantification of relative protein levels from western blots. Data are depicted as mean +/- SD, n = 3, **P < 0.01, ***P < 0.001 and ****P < 0.0001 by one-way ANOVA. Intracellular transport, activation, mitochondrial transport, B Beta-oxidation, carnitine shuttle, and auxiliary proteins. The primary antibodies used as follows: VLCAD 1:1000, MCAD 1:1000, LCAD 1:1000, TFPa 1:500, TFPb 1:3000, CPT1A Alpha 1:1000, and GAPDH 1:30,000 dilutions overnight at 4 °C. The membranes were then incubated with fluorescent conjugated secondary antibodies for 1 h; DyLight 800 conjugated goat Anti-Rabbit IgG (611-145-002), DyLight 680 conjugated goat Anti-Rabbit IgG (611-144-003), DyLight 800 conjugated goat Anti-Mouse IgG (610-145-002), and DyLight 680 conjugated donkey Anti-Mouse IgG (610-744-124). Fig 1. PMID: 33725513.
Assessment of mitochondrial fusion and fission. B. Representative western blots (original blots are shown in supplementary Fig. S10) and quantification of MFN1/2 and DRP1. No significant changes in the relative levels of proteins that facilitate mitochondrial fusion (MFN1/2) and fission (DRP1) between non-disease (control) and mutant primary fibroblasts. Data are depicted as mean +/- SD, n = 3. The primary antibodies used as follows: MFN1 1:400, MFN2 ( 1:400, DRP1 1:100 and GAPDH 1:30,000 dilutions overnight at 4 °C. The membranes were then incubated with fluorescent conjugated secondary antibodies for 1 h; DyLight 800 conjugated goat Anti-Rabbit IgG (611-145-002), Antibody DyLight 680 conjugated Anti-Rabbit IgG made in goat (611-144-003), DyLight 800 conjugated goat Anti-Mouse IgG (610-145-002), and DyLight 680 conjugated donkey Anti-Mouse IgG (610-744-124). Fig 3. PMID: 33725513.
Properties of DyLight™ Conjugates.
DyLight™ dyes can be used for two-color Western Blot detection with low background and high signal. Anti-tubulin was detected using a DyLight™ 680 conjugate. Anti-TNFa was detected using a DyLight™ 800 conjugate. The image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.