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Antibodies

Rabbit IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Product Sizes
2 mg
611-1322-2MG
About this Product
SKU:
611-1322
Additional Names:
goat anti-Rabbit IgG Antibody peroxidase conjugated, Gt-a-Rabbit peroxidase conjugated IgG, Rabbit peroxidase conjugated Antibody in Goat, Rabbit Secondary peroxidase conjugated Antibody, HRP secondary, peroxidase secondary
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
HRP
Concentration:
2 mg/ml
Extra Details:
Anti-Rabbit IgG peroxidase conjugated antibody generated in goat detects specifically rabbit IgG. Both the Heavy and Light chains of the antibody molecule are present. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG is conjugated with Peroxidase. This peroxidase conjugated anti-Rabbit secondary antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Rabbit IgG whole molecule
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Rabbit
Shipping Conditions:
Ambient
Specificity:
Anti-RABBIT IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No re
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
Western Blot of Unconjugated Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) (p/n 611-101-122). Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) (p/n 611-101-122) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
Western Blot of Goat anti-Rabbit IgG Peroxidase Conjugated Antibody. Lane 1: HeLa Whole Cell Lysate. Lane 2: NIH 3T3 Whole Cell Lysate. Load: 10 ug per lane. Primary antibody: Beta Actin antibody at 1:2,000 for overnight at 4°C. Secondary antibody (2 Blots on Left): Atto 647N goat secondary antibody at 1:10,000 for 60 min at RT. Secondary Antibody (2 Blots on Right): Peroxidase goat secondary antibody at 1:10,000 for 60 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 50 kDa, 50 kDa for Beta Actin. Other band(s): none.