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Antibodies

Rabbit IgG Fc Antibody Peroxidase Conjugated

Product Sizes
2 mg
£234.00
611-1303-2MG
About this Product
SKU:
611-1303
Additional Names:
Goat anti-Rabbit IgG F(c) Antibody Peroxidase Conjugation, Goat anti-Rabbit IgG Fc fragment Antibody peroxidase Conjugation, Goat anti-Rabbit IgG F(c) HRP Conjugated Antibody
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
HRP
Concentration:
2 mg/ml
Extra Details:
Anti-Rabbit IgG F(c) peroxidase antibody generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of rabbit IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Anti-Rabbit IgG F(c) fragment was produced by repeated immunization with Rabbit IgG F(c) fragment in goat.
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Rabbit
Shipping Conditions:
Ambient
Specificity:
Anti-Rabbit IgG F(c) antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against Rabbit IgG F(ab).|IgG Fc
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles., -20[o]C/-70[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
FluoroFinder:
Western Blot of Anti-Rabbit IgG F(c) (GOAT) Antibody (p/n 611-1103). Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng of IgG, F(ab), IgM and Serum, 100 ng of F(c). Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG F(c) (GOAT) Antibody (p/n 611-1103) 1:2,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
Western blot of Peroxidase conjugated Goat Anti-Rabbit IgG F(c) secondary antibody. Lane 1: Rabbit IgG F(c). Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for Rabbit IgG F(c). Other band(s): None.