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Antibodies

Rabbit IgG Fc Antibody Pre-Adsorbed

Product Sizes
2 mg
£181.00
611-1123-2MG
About this Product
SKU:
611-1123
Additional Names:
goat anti-Rabbit F(c) Antibody, Gt-a-Rabbit IgG F(c), Rabbit Antibody in Goat, Goat anti-Rabbit F(c) fragment Secondary Antibody
Application:
Dot Blot, ELISA, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
2 mg/ml
Extra Details:
Anti-Rabbit IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of rabbit IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Anti Rabbit IgG F(c) was produced by repeated immunization with rabbit IgG F(c) fragment in goat.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Rabbit
Shipping Conditions:
Blue Ice
Specificity:
IgG Fc|This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against Rabbit IgG F(ab) o
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
Western Blot of Anti-Rabbit IgG F(c) (GOAT) Antibody (p/n 611-1103). Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng of IgG, F(ab), IgM and Serum, 100 ng of F(c). Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG F(c) (GOAT) Antibody (p/n 611-1103) 1:2,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
Dot Blot Results of Goat Anti-Rabbit F(c) mx Human IgG. Row A: Rabbit F(c) protein (p/n 011-0103). Row B: Rabbit F(ab) protein (p/n 011-0105). Row C: Human IgG protein (009-0102). Amount Dotted: (1) 10ng, (2) 3.33ng, (3) 1.11ng, (4) 0.37ng, (5) 0.12ng. Primary Antibody: Anti-Rabbit F(c) at 1.0uL/mL for 1hr at RT. Secondary Antibody: Donkey Anti-Goat IgG Peroxidase Conjugate at 1:40,000 for 30mins at RT. Block: BlockOut Buffer (p/n MB-073). Exposure: 60secs.
Dot Blot of Goat Anti-RABBIT IgG F(c) Antibody (Min X Human Serum Proteins). Row A: Rabbit IgG whole Molecule (p/n 011-0102). Row B: Rabbit F(c) (p/n 011-0103). Row C; Rabbit F(ab) (p/n -011-0105). Samples Loaded: 1- 100ng, 2- 33.33ng, 3- 11.11ng, 4- 3.70ng, 5- 1.23ng. Primary Antibody: Goat Anti-RABBIT IgG F(c) Mx Hu at 1.0ug/mL for 60mins at RT. Secondary Antibody: Custom HRP at 1:40,000 for 30mins at RT. Blocking (p/n MB-070) for 60 mins at RT. Exposure: 5 sec.
ELISA results of purified Goat Anti-Rabbit IgG F(c) Antibody (Min x Human serum proteins) tested against purified Rabbit IgG F(c) (Red Line), Rabbit IgG (Green Line), and Human IgG (Blue Line). Each well was coated in duplicate with 1.0 ug of antigen Rabbit IgG F(c) (011-0103), Rabbit IgG (011-0102), and Human IgG (009-0102). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-060-1000), Donkey Anti-Goat IgG HRP (p/n 605-703-125), and TMB substrate (p/n TMBE-1000).
Western Blot of Goat anti-Rabbit Fc antibody. Lane 1: Rabbit Fc (p/n 011-0103). Load: 100 ng per lane. Primary antibody: Rabbit Fc antibody at 1:1,000 for overnight at 4°C. Secondary antibody: DyLight™ 649 goat secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for Rabbit Fc. Other band(s): Rabbit Fc splice variants and isoforms.