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Antibodies

Rabbit IgG (H&L) Antibody Phycoerythrin conjugated Pre-Adsorbed

Product Sizes
1 ml
£310.00
611-108-122-1ML
About this Product
SKU:
611-108-122
Additional Names:
goat anti-Rabbit IgG Antibody, Gt-a-Rabbit Phycoerythrin conjugated, Rabbit IgG (H&L) Antibody in goat, Goat IgG (H&L) Phycoerythrin conjugated Secondary Antibody.
Application:
Flow Cytometry, Immunofluorescence, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
R-PE
Concentration:
0.5 mg/ml
Extra Details:
Anti-Rabbit IgG (H&L) phycoerythrin conjugated antibody generated in goat detects specifically rabbit IgG. This secondary phycoerythrin conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, microscopy and FACS analysis.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Anti-Rabbit IgG was produced by repeated immunization with rabbit IgG whole molecule in goat.
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Rabbit
Shipping Conditions:
Ambient
Specificity:
IgG (H&L)|Rabbit IgG (H&L) Antibody Phycoerythrin conjugated Pre-Adsorbed was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, conjugation to phycoerythrin and subsequent purification, and extensive dialysis against the buffer stated above. Assay by immunoelectr
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
FluoroFinder:
Western Blot of Unconjugated Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) (p/n 611-101-122). Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) (p/n 611-101-122) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
Western Blot of Goat anti-Rabbit IgG Pre-Absorbed Phycoerythrin Conjugated Antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Phycoerythrin goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 28 & 55 kDa, 55 kDa for Rabbit IgG. Other band(s): None.