Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Rabbit IgG (H&L) Antibody CY3 Conjugated Pre-adsorbed

Product Sizes
1 mg
611-104-122-1MG
About this Product
SKU:
611-104-122
Additional Names:
goat anti-Rabbit IgG CY3 Conjugated antibody, goat anti-Rabbit IgG antibody CY3 conjugation
Application:
Detection/Quantitation, Dot Blot, FLISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
Cyanine 3
Concentration:
1 mg/ml
Extra Details:
Anti-Rabbit IgG CY3 Conjugated Antibody generated in goat detects rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG (H&L) is conjugated to Cy3.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Rabbit IgG whole molecule
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Rabbit
Shipping Conditions:
Ambient
Specificity:
IgG (H&L)|RABBIT IgG (H&L) Antibody CY3 Conjugated Pre-adsorbed was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
Western Blot of Unconjugated Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) (p/n 611-101-122). Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) (p/n 611-101-122) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
Dot Blot Results of Goat Anti-Rabbit IgG CY3 Conjugated Antibody Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins. Rabbit IgG (p/n 011-0102) samples 1-100ng, 2- 33.3ng, 3- 11.1ng, 4- 3.70ng, 51.23ng. Goat Anti-Rabbit IgG CY3 Conjugated Antibody at 1.0ug/mL for 30mins at RT. Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) for 60mins at RT.
Western Blot of Goat anti-Rabbit IgG Pre-Absorbed Cy3 Conjugated Secondary Antibody. Lane 1: Rabbit IgG. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Cy3 goat anti-rabbit secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Rabbit IgG. Other band(s): None.