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Antibodies

Rabbit IgG Fc Antibody Rhodamine Conjugated

Product Sizes
2 mg
611-1003-2MG
About this Product
SKU:
611-1003
Additional Names:
Goat anti-Rabbit IgG F(c) Antibody Rhodamine Conjugation, Goat anti-Rabbit IgG Fc fragment Antibody Rhodamine Conjugation, Goat anti-Rabbit IgG F(c) Rhodamine Conjugated Antibody
Application:
Electron Microscopy, FLISA, Flow Cytometry, Immunofluorescence
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
TRITC
Concentration:
2 mg/ml
Extra Details:
Anti-Rabbit IgG F(c) Rhodamine antibody generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of rabbit IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Rabbit IgG F(c) fragment
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Rabbit
Shipping Conditions:
Ambient
Specificity:
IgG Fc|This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against Rabbit IgG F(ab).
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
(a) Channels coating: remnant fluorescence due to nonspecific binding of anti-rabbit IgG labeled with B-rhodamine (p/n 611-1003) [AbII] at a concentration of 100 ug/ml in uncoated and coated microchannels with silane-PEG. (b) Fluorescence obtained from the complete immunoassay at a flow rate of 5 ul/h and at a anti-biotin rabbit IgG (p/n 100-4198) [AbI] concentration of 50 pg/ml is compared to the fluorescence obtained from the immunoassay performed without applying the magnetic field (column 1), without adding anti-biotin rabbit IgG (p/n 100-4198) [AbI] (column 2), without adding ), anti-rabbit IgG labeled with B-rhodamine (p/n 611-1003) [AbII] (column 3), and, finally, the efficacy of the microparticles coating was tested by performing the immunoassay without anti-biotin rabbit IgG (p/n 100-4198) [AbI] and with noncoated microparticles (column 5). The level of fluorescence of the two first columns in b results from nonspecific interactions of anti-rabbit IgG labeled with B-rhodamine (p/n 611-1003) [AbII] with the microparticles. Error bars are standard deviation. FIG. 5. PMID: 32038740.