Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Rabbit IgG (H&L) Antibody Rhodamine Conjugated Pre-Adsorbed

Product Sizes
1 mg
£259.00
611-100-122-1MG
About this Product
SKU:
611-100-122
Additional Names:
Goat anti-Rabbit IgG Antibody Rhodamine Conjugation, Goat anti-Rabbit IgG Rhodamine Conjugated Antibody
Application:
Detection/Quantitation, FLISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
TRITC
Concentration:
1 mg/ml
Extra Details:
Anti-Rabbit IgG Antibody Rhodamine generated in goat detects rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG (H&L) is conjugated to Rhodamine.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Rabbit IgG whole molecule
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Rabbit
Shipping Conditions:
Ambient
Specificity:
IgG (H&L)|This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pi
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles., -20[o]C/-70[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
FluoroFinder:
Double immunofluorescence images of glucagon-like peptide-1 receptor (GLP-1R, a, d, g) and somatostatin (SOM, b, e, h) in the pancreatic islets of chickens (a-c), northern bobwhites (d-f), and ostriches (g-i). Figures c, f, and i show merged images of a and b, d and e, and g and h, respectively. Almost every SOM-immunoreactive cell in the pancreatic islets of three avian species also demonstrated GLP-1R immunoreactivity. Bars indicate 10 um. Fig. 1. PMID: 32055175.
Merged images of double immunofluorescence pictures of glucagon-like peptide-1 receptor (red) and insulin (green) (GLP-1R + INS, a-c), and glucagon-like peptide-1 receptor (red) and glucagon (green) (GLP-1R + GLU, d-f) in the pancreatic islets of chickens (a and d), northern bobwhites (b and e), and ostriches (c and f). Islet cells showing either insulin or glucagon immunoreactivity were immunonegative to glucagon-like peptide-1 receptor. Bars indicate 10 um. Fig. 2. PMID: 32055175.
Fluorescent micrographs of MCF-7 cells unexposed to either solvent (a) exposed to 0.5% Methanol (b) and 0.5% DMSO (c). D is negative control. BCL-2 was stained with monoclonal anti-BCL-2 and Goat Anti-Mouse IgG FITC conjugated (Upper Right Quadrant- showing the nuclear and cytoplasmic expression of the oncogene BCL-2). BAX was stained with polyclonal rabbit anti-BAX and Goat anti-Rabbit IgG Rhodamine conjugated (Lower Left quadrant- showing the nuclear and cytoplasmic expression of BAX). Nucleus was counterstained with DAPI (Upper left quadrant). The lower right quadrant shows the combined image. 2D shows fluorescent micrographs of MCF-7 cells unexposed to either solvent. Cells were incubated with the secondary antibodies but not the primary antibodies to reveal non-specific staining. The nucleus was counterstained with DAPI (Upper left quadrant). The lower right quadrant represents the combined image. Figure 2. PMID: 26229223.