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Antibodies

Mouse IgG (H&L) Antibody DyLight[TM] 405 Conjugated Pre-Adsorbed

Product Sizes
100 ug
610-746-124-100UG
About this Product
SKU:
610-746-124
Additional Names:
Donkey anti-Mouse IgG DyLight 405™ Conjugated Antibody, Donkey anti Mouse IgG Antibody DyLight 405™ Conjugation
Application:
FLISA, Immunofluorescence, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
DyLight™ 405
Concentration:
1 mg/ml
Extra Details:
Anti-Mouse IgG DyLight405 Antibody generated in donkey detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Donkey
Immunogen:
Mouse IgG whole molecule
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Mouse
Shipping Conditions:
Ambient
Specificity:
IgG (H&L)|This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
Double-immunofluorescence staining for aquaporin-2 (green) and calbindin (a marker of connecting tubules, red) demonstrates the sudden transition from a calbindin-positive connecting tubule to an aquaporin-2-positive collecting duct cyst (asterisk in D). Scale bars: 100 um. Fig. 7. PMID: 34345895.
Intrarenal distribution of polycystin-2poreL1 in homozygous Pkd2poreL1 knock-in mice. (A,B) Immunofluorescence staining for aquaporin-2 as a marker for collecting ducts on the one hand and for polycystin-2 and polycystin-2poreL1 on the other hand in 6-month-old wild-type (+/+) and homozygous Pkd2poreL1 knock-in (p/p) female mice demonstrates the presence of wild-type polycystin-2 and of polycystin-2poreL1 in papillary collecting ducts in both the C57Bl/6 and 129/Sv genetic backgrounds. Note the larger diameter of collecting ducts in the Pkd2poreL1/poreL1 mice. Scale bar: 50 um. (C,D) Immunofluorescence staining of kidney sections from 6-month-old wild-type (+/+) and homozygous Pkd2poreL1 knock-in (p/p) mice. Both in the C57Bl/6 and in the 129/Sv background, identical distributions of the wild-type and mutant polycystin-2 proteins (red signal) are seen. Nuclei are shown in white. Images are representative of three experiments. Scale bars: 10 um. Fig. 5. PMID: 34345895.
Properties of DyLight™ Fluorescent Dyes.