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Antibodies

Mouse IgG2a (Gamma 2a chain) Antibody Texas Red[TM] Conjugated

Product Sizes
1 mg
£245.00
610-4941-1MG
About this Product
SKU:
610-4941
Additional Names:
Rabbit Anti-Mouse IgG2a (Gamma 2a chain) Antibody Texas Red™ Conjugated, Rabbit Anti Mouse IgG2a Texas Red™ Conjugated Antibody
Application:
Detection/Quantitation, FLISA, Flow Cytometry, Immunofluorescence, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
Texas Red®
Concentration:
1 mg/ml
Extra Details:
Anti-Mouse IgG2a Texas Red Antibody generated in rabbit detects reactivity to Mouse IgG2a (Gamma 2a chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25% of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Mouse IgG2a heavy chain
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Mouse
Shipping Conditions:
Ambient
Specificity:
IgG2a|This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Mouse Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles., -20[o]C/-70[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
FluoroFinder:
Western blot of Texas Red™ conjugated Rabbit Anti-Mouse IgG2a (Gamma 2a chain) secondary antibody. Lane 1: Mouse IgG2a. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red™ rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG2a (Gamma 2a chain). Other band(s): None.
Isolated hADSCs (IADSCs) were differentiated into SMCs using retinoic acid (RA), heparin was used as a positive control, while SK-UT-1 cells was used as a SMC control. Expression of SMC markers smooth muscle alpha actin (SM-A Alphaa, red, Texas Red Conjugated anti-Mouse IgG2, G Gamma2a chain specific, p/n 610-4941), desmin (green, Fluorescein Conjugated anti-Mouse IgG1, G Gamma1 chain specific, p/n 610-4240), smooth muscle myosin heavy chain (SM-MHC, red, Texas Red Conjugated anti-Mouse K Kappa, kappa chain specific, p/n 610-4910), and smoothelin (green, Fluorescein Conjugated anti-Mouse IgG1, G Gamma1 chain specific, p/n 610-4240) in differentiated SMCs was determined by indirect immunofluorescence. Nuclei were counter stained with DAPI (blue). Expression of all four markers can be seen in all the cells, particularly in RA differentiated SMCs. Fig. 5. PMID: 21373882.