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Antibodies

Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody ATTO 550 Conjugated

Product Sizes
100 ug
610-454-C46S-100UG
About this Product
SKU:
610-454-C46S
Additional Names:
rabbit anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody ATTO550 conjugation, rabbit anti-Mouse IgG ATTO550 conjugated Antibody
Application:
FLISA, Immunofluorescence, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
ATTO 550
Concentration:
1 mg/ml
Extra Details:
Anti-Mouse IgG ATTO 550 Antibody generated in rabbit detects reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. IgG1, IgG2a, IgG2b and IgG3 chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Mouse
Shipping Conditions:
Ambient
Specificity:
IgG (gamma 1, 2a, 2b and 3 chain)|Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody ATTO 550 was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG clas
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
ATTO ® dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Joerg Reichwein, ATTO-TEC GmbH
Western Blot of ATTO 550 Rabbit Anti-Mouse IgG (gamma 1, 2a, 2b, 3) secondary antibody. Lane 1: Mouse IgG1. Lane 2: Mouse IgG2a. Lane 3: Mouse IgG2b. Lane 4: Mouse IgG3. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: ATTO 550 rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG Subclasses. Other band(s): None.
Atto™ dyes can be used for multicolor immunofluorescent detection with low background and high signal. Example shown here is Immunohistochemical staining using ATTO-550 Anti-Aquaporin 2-antibody (red) of paraffin embedded region of rat kidney showing a transversal cut of the inner medulla near to the renal papilla. Nuclei are visualized with Hoechst 33342 (blue). Images provided courtesy of Dr. Joerg Reichwein, ATTO-TEC GmbH