Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Mouse IgG Fc Antibody Alkaline Phosphatase Conjugated

Product Sizes
1 mg
£272.00
610-4503-1MG
About this Product
SKU:
610-4503
Additional Names:
Rabbit Anti-Mouse IgG F(c) Antibody alkaline phosphatase Conjugation, Rabbit Anti-Mouse IgG Fc Antibody alkaline phosphatase Conjugated, Rabbit Anti-Mouse IgG Fc Fragment alk phos Conjugated Antibody
Application:
Dot Blot, ELISA, Immunohistochemistry, Western Blot
Buffer:
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
AP
Concentration:
1 mg/ml
Extra Details:
Anti-Mouse IgG F(c) generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of mouse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
Formulation:
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Host:
Rabbit
Immunogen:
Mouse IgG F(c) fragment
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Mouse
Shipping Conditions:
Blue Ice
Specificity:
IgG Fc|This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reacti
Storage Conditions:
2-8[o]C unopened. Do not freeze.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
ELISA Results of Rabbit Anti-Mouse IgG F(c) Antibody Alkaline Phosphatase Conjugated tested against purified Mouse IgG F(c) Alk Phos. Each well was coated in duplicate with 1.0 ug of Mouse IgG F(c) (p/n 010-0103). The working dilution is 1: 12,500. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugated Stabilizer (p/n MB-076), ELISA Alkaline Phosphatase Conjugation Buffer (p/n NPP-10), NPP Working Buffer (p/n NPP-B500).
ELISA and SDS-PAGE analysis under thermal stress. (a,c,e) ELISA of the antigen-binding activity of 6C407-derived antibodies. Purified 6C407 antibodies were heated under the specified conditions, placed in wells coated with KIFC143-54 peptide (EDGLEPEKKRTR), and bound 6C407 antibodies were detected with AP-conjugated antibodies specific for mouse IgG/Fc (a), human IgG/Fc (b), or chicken IgY/U Upsilon chain (c). Data are presented as mean +/- SD (n = 3). (b,d,f) SDS-PAGE analysis of antibody integrity. Purified 6C407 antibodies were heated under the specified conditions then subjected to SDS-PAGE under reducing conditions using a 12% polyacrylamide gel, followed by staining with Coomassie Blue. Figure provided by CiteAb. Source: Sci Rep, PMID: 31848417.
ELISA and SDS-PAGE analysis under thermal stress. (a,c,e) ELISA of the antigen-binding activity of 3D8 antibodies. Purified 3D8-derived antibodies were heated under the specified conditions, placed in wells coated with plasmid DNA antigen (pUC19), and bound 3D8 antibodies were detected with AP-conjugated antibodies specific for mouse IgG/Fc (a), human IgG/Fc (b), or chicken IgY/U Upsilon chain (c). Data are presented as mean +/- SD (n = 3). (b,d,f) SDS-PAGE analysis of antibody integrity. Purified 3D8 antibodies were heated under the specified conditions then subjected to SDS-PAGE under reducing conditions using a 12% polyacrylamide gel, followed by staining with Coomassie Blue. Figure provided by CiteAb. Source: Sci Rep, PMID: 31848417.