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Antibodies

Mouse IgG3 (Gamma 3 chain) Antibody DyLight[TM] 549 Conjugated

Product Sizes
100 ug
£173.00
610-442-043-100UG
About this Product
SKU:
610-442-043
Additional Names:
Rabbit Anti-Mouse IgG3 (Gamma 3 chain) DyLight 549™ Conjugated Antibody, Rabbit Anti Mouse IgG3 Antibody DyLight 549™ Conjugation
Application:
Detection/Quantitation, FLISA, Immunofluorescence, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
DyLight™ 549
Concentration:
1 mg/ml
Extra Details:
Anti-Mouse IgG3 DyLight 549 Antibody generated in rabbit detects reactivity to Mouse IgG3 (Gamma 3 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. IgG3 comprises almost 10% of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Mouse IgG3 heavy chain
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Mouse
Shipping Conditions:
Ambient
Specificity:
IgG3|This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum. Minimal cross reactivity was noted against other Mouse immunoglobulin cl
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles., -20[o]C/-70[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
FluoroFinder:
Properties of DyLight™ Conjugates.
DyLight™ dyes can be used for two-color Western Blot detection with low background and high signal. Anti-tubulin was detected using a DyLight™ 549 conjugate. Anti-TNFa was detected using a DyLight™ 649 conjugate. The image was captured using the Typhoon™ 9410 Imaging System.
Homology modelling and topology of human CASD1. (b) Orientation of the N- and C-terminus of CASD1. LM-TK- cells expressing CASD1 with an N-terminal V5-tag and a C-terminal Myc-tag (V5-CASD1-Myc) were fixed and permeabilized with Triton X-100 (0.2%) or digitonin (5 ug ml-1). Cells were stained with anti-V5 or anti-Myc mAb (red) in combination with anti-A Alpha-mannosidase II (A Alpha-Man II) pAb (green) recognizing the luminal domain of the Golgi marker A Alpha-Man II. Nuclei were counterstained with DAPI (blue). Figure 1. PMID: 26169044.