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Antibodies

Mouse IgG2b (Gamma 2b chain) Antibody Peroxidase Conjugated

Product Sizes
1 mg
610-4342-1MG
About this Product
SKU:
610-4342
Additional Names:
Rabbit Anti-Mouse IgG2b (gamma 2b chain) Antibody peroxidase Conjugated, Rabbit Anti-Mouse IgG2b Antibody HRP Conjugation
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
HRP
Concentration:
1 mg/ml
Extra Details:
Anti-Mouse IgG2b peroxidase conjugated antibody generated in rabbit detects specifically mouse IgG2b. This secondary peroxidase conjugated antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-Mouse IgG2b was produced by repeated immunization with mouse IgG2b heavy chain in rabbit.
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Mouse
Shipping Conditions:
Ambient
Specificity:
IgG2b|This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA at less than 1% cr
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
ELISA Results of Rabbit Anti-Mouse IgG2b Antibody Peroxidase Conjugation tested against purified Mouse IgG2b HRP. Each well was coated in duplicate with 1.0 ug of Mouse IgG2b (p/n 010-0142). The working dilution is 1:82,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate (p/n TMBE-1000).
Induction of HBV core-specific antibodies in B6 and TLR7-/- mice. B6 and TLR-7-/- (n = 4/4) were immunized with recombinant HEK-293-derived stC149tat particles. Serum samples were obtained and analysed as described in the M&M section. Mean specific antibody titers in sera +/-SD (a) and the calculated IgG1/IgG2a ratios +/-SD (b) of a representative experiment (out of two performed experiments) are shown. The statistical significance of differences in IgG and IgG2b antibody titers between stC149tat immune B6 and TLR7-/- mice was determined by the unpaired Student's t-test. P values of <0.05 (*) and <0.001 (***) were considered statistically significant. Figure provided by CiteAb. Source: Sci Rep, PMID: 30279478.
Characterization of antibody responses induced by DNA vaccines expressing particulate and non-particulate core antigens. (a-f) Mice were immunized intradermally with 2 ug particle-coated plasmids with the gene gun (see Supplemental protocols). At d21 mice were boosted with the same vectors. The specific serum Ab responses and isotype profiles (IgG, IgG1, IgG2a) were determined 12 days post boost immunization by end-point dilution ELISA using bacterial rHBV-C149 particles as detection antigen and IgG1/IgG2a ratios were calculated. (a,b) B6 mice (n = 3/4) were immunized with pCI/stC149tat or pCI/stC149 vectors. (c,d) B6 and TLR7-/- mice (n = 3/5) were immunized with pCI/stC149tat. (e,f) B6 mice (n = 5/5) were immunized with pCI/stCAAA or pCI/stCAAAY132A plasmid DNA. Mean specific antibody titers in sera (a,c,e) and the calculated IgG1/IgG2a ratios +/-SD (b,d,f) of representative experiments (out of two experiments performed) are shown. Where indicated, the statistical significance of differences in IgG, IgG1 and IgG2b antibody titers was determined by the unpaired Student's t-test. P values of < 0.05 (*) and < 0.005 (**) were considered statistically significant. Figure provided by CiteAb. Source: Sci Rep, PMID: 30279478.
Induction of HBV core-specific antibodies in mice. (a) B6 mice were immunized with recombinant HEK-293-derived stC149tat or stC149 (n = 4/5). Three weeks post injection serum samples were obtained by tail bleeding and HBV core-specific IgG, IgG1 and IgG2b serum antibody titers were determined by end-point dilution ELISA using bacterial rHBV-C149 particles as detection antigen. Mean specific antibody titers in sera +/-SD (a) and the calculated IgG1/IgG2a ratios +/-SD (b) of a representative experiment (out of two performed experiments) are shown. The statistical significance of differences in IgG, IgG1 and IgG2b antibody titers between stC149tat- and stC149 immune B6 mice were determined by the unpaired Student's t-test. (c) B6 mice were immunized with recombinant stC149tat or stC149 proteins. Ten days post immunization spleen cells were stimulated ex vivo for 2 days with the HBV-Core-specific I-Ab-binding C128-140 peptide. The specific IFN-G Gamma release into the cell culture supernatants was determined by ELISA. The statistical significance of differences in IFN-G Gamma levels between stC149- and stC149tat-immune mice (groups 2 and 3) were determined by the unpaired Student's t-test. (a-c) P values of <0.05 (*) and <0.01 (**) were considered statistically significant. Figure provided by CiteAb. Source: Sci Rep, PMID: 30279478.
Characterization of antibody responses induced by DNA vaccines expressing particulate and non-particulate core antigens. (a-f) Mice were immunized intradermally with 2 ug particle-coated plasmids with the gene gun (see Supplemental protocols). At d21 mice were boosted with the same vectors. The specific serum Ab responses and isotype profiles (IgG, IgG1, IgG2a) were determined 12 days post boost immunization by end-point dilution ELISA using bacterial rHBV-C149 particles as detection antigen and IgG1/IgG2a ratios were calculated. (a,b) B6 mice (n = 3/4) were immunized with pCI/stC149tat or pCI/stC149 vectors. (c,d) B6 and TLR7-/- mice (n = 3/5) were immunized with pCI/stC149tat. (e,f) B6 mice (n = 5/5) were immunized with pCI/stCAAA or pCI/stCAAAY132A plasmid DNA. Mean specific antibody titers in sera (a,c,e) and the calculated IgG1/IgG2a ratios +/-SD (b,d,f) of representative experiments (out of two experiments performed) are shown. Where indicated, the statistical significance of differences in IgG, IgG1 and IgG2b antibody titers was determined by the unpaired Student's t-test. P values of < 0.05 (*) and < 0.005 (**) were considered statistically significant. Figure provided by CiteAb. Source: Sci Rep, PMID: 30279478.