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Antibodies

Mouse IgG2a Antibody

Product Sizes
1 mg
£210.00
610-4141-1MG
About this Product
SKU:
610-4141
Additional Names:
Rabbit Anti-Mouse IgG2a Antibody, Mouse IgG2a Secondary Antibody, Rb-a-mouse IgG2a
Application:
Dot Blot, ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1.02 mg/ml
Extra Details:
Anti-MOUSE IgG2a (Gamma 2a chain) (RABBIT) Antibody generated in rabbit detects specifically Mouse IgG2a heavy chain. Anti-Mouse IgG2a is ideal for investigators involved in Serum Protein Component research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Mouse IgG2a heavy chain
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Mouse
Shipping Conditions:
Blue Ice
Specificity:
Anti-MOUSE IgG2a (Gamma 2a chain) (RABBIT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG2a coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse Serum, Mouse IgG and Mouse IgG2a. Specificity was confir
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
Western Blot of Rabbit anti-Mouse IgG2a antibody. Lane 1: Mouse IgG1 (p/n 010-0102). Lane 2: Mouse IgG2a (p/n 010-0141). Lane 3: Mouse IgG2b (p/n 010-0142). Lane 4: Mouse IgG3 (p/n 010-0143). Lane 5: Mouse IgM (p/n 010-0107). Load: 5 ng per lane. Primary antibody: Mouse IgG2a antibody at 1 ug/mL for overnight at 4°C. Secondary antibody: Gt-anti-Rabbit HRP (p/n 611-103-122) at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 50 kDa and25 kDa, 50 kDa and 25 kDa for Mouse IgG2a. Other band(s): Weak reactivity for to Mouse IgG1.
Dot Blot of Rabbit Anti-Mouse IgG2a Antibody. Tested with dilutions 10ng, 3.3ng, 1.1ng, 0.37ng, 0.12ng. Lane 1: Mouse IgG1 (p/n 010-0102). Lane 2: Mouse IgG2a (p/n 010-0141). Lane 3: Mouse IgGb (p/n 010-0142). Lane 4: Mouse IgG3 (p/n 010-0143). Lane 5: Mouse IgM (p/n 010-0107). Primary Antibody: Rabbit anti-Mouse IgG2a at 1ug/mL for 1 hour at RT. Secondary Antibody: Gt-anti-Rabbit HRP (p/n 611-103-122) at 1:40,000. Blocking Buffer: BlockOut MB-073 for 30 min at RT.