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Antibodies

Mouse IgG1 Antibody

Product Sizes
1 mg
£210.00
610-4140-1MG
About this Product
SKU:
610-4140
Additional Names:
Rabbit Anti-Mouse IgG1 (Gamma 1 chain) Antibody, Rabbit Anti-Mouse IgG1 Antibody
Application:
Dot Blot, ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1.14 mg/ml
Extra Details:
Anti-Mouse IgG1 Antibody generated in rabbit detects reactivity to Mouse IgG1 (Gamma 1 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. IgG1 chain constitutes 66% of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Mouse IgG1 heavy chain
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Mouse
Shipping Conditions:
Blue Ice
Specificity:
Anti-Mouse IgG1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse Serum, Mouse IgG and Mouse IgG1. Specificity was confirmed by ELISA. For IgG2a, IgG2b
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
ELISA results of purified Rabbit anti-Mouse IgG1 tested against purified Mouse IgG1. Each well was coated in duplicate with 1.0 ug of Mouse IgG1 (p/n 010-0141). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-060-1000), Goat anti-Rabbit HRP (p/n 611-103-122), and TMB substrate (p/n TMBE-1000).
Dot Blot of Rabbit Anti-Mouse IgG1 Antibody. Tested against dilutions 10ng, 3.3ng, 1.1ng, 0.37ng, 0.12ng. Lane 1: Mouse IgG1 (p/n 010-0140). Lane 2: Mouse IgG2a (p/n 010-0141). Lane 3: Mouse IgG2b (p/n 010-0142). Lane 4: Mouse IgG3 (p/n 010-0143). Lane 5: Mouse IgM (p/n 610-1107). Primary Antibody: Rabbit anti-Mouse IgG1 at 1ug/mL for 1 hour at RT. Secondary Antibody: Goat anti-Rabbit HRP (p/n 611-103-122) at 1:40,000. Blocking Buffer: BlockOut (p/n MB-073) for 30 min at RT.