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Antibodies

Mouse IgG (H&L) Secondary Antibody

Product Sizes
2 mg
£151.00
610-4102-2MG
Academic Pricing
Please contact us for academic pricing.
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About this Product
SKU:
610-4102
Additional Names:
rabbit anti-Mouse IgG Antibody, rabbit anti-mouse IgG heavy and light chain antibody, rb anti-ms IgG heavy and light chain, Anti-Mouse Secondary Antibody, rabbit anti Mouse IgG Antibody
Application:
Electron Microscopy, ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
TR
Concentration:
2.08 mg/ml
Extra Details:
Anti-Mouse IgG Antibody generated in rabbit detects reactivity to Mouse IgG heavy and light chains. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Format:
IgG
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Mouse IgG whole molecule
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Mouse
Shipping Conditions:
Blue Ice
Specificity:
Anti-Mouse IgG (H&L) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum.|IgG (H&L)
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Size:
2 mg
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
FcG GammaRIV-dependent uptake and activation of kidney, but not dermal, macrophages by small immune complexes, 'auto' antibodies, and NZWxBXSB serum. (H) TNF staining in kidney F4/80bright macrophages from wt mice 1 hour after i.v. injection of the indicated amounts of BSA, RaBSA, BSA;RaBSA, rabbit IgG (Ra-IgG), rabbit anti-mouse transferrin (Ra-mTransferrin) [p/n 600-401-255], rabbit anti-mouse albumin (Ra-mAlbumin) [p/n 600-401-254], rabbit anti-mouse IgG (Ra-mIgG) [p/n 610-4102] or Ra- mIgG (Fab')2 [p/n 310-4102]. N= 4-5 mice per treatment. Fig 5. PMID: 27477514.
ELISA results of purified Rabbit anti-Mouse IgG Antibody tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 ug of Mouse IgG (p/n 010-0102). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin (p/n MB-067) as blocking buffer, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n 611-103-122) and TMB substrate (p/n TMBE-1000).