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Antibodies

Mouse IgG (H&L) Antibody DyLight[TM] 680 Conjugated Pre-Adsorbed

Product Sizes
100 ug
610-144-121-100UG
About this Product
SKU:
610-144-121
Additional Names:
Goat Anti-Mouse IgG Secondary Antibody DyLight™680 Conjugated, Goat Anti-Mouse IgG Antibody DyLight™680 Conjugated, Anti-mouse IgG secondary antibody, anti-mouse IgG DyLight™680 conjugated secondary antibody
Application:
Detection/Quantitation, FLISA, Immunofluorescence, Immunohistochemistry, Microarray, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
DyLight™ 680
Concentration:
1 mg/ml
Extra Details:
Anti-Mouse IgG DyLight 680 Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Mouse IgG whole molecule
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Mouse
Shipping Conditions:
Ambient
Specificity:
IgG (H&L)|This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig,
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
Peptide array results using Goat Anti-Mouse IgG (H&L) Antibody DyLight™ 680 Conjugated. Peptide arrays identify known immunogenic epitopes in L1. (A) Synthetic 15-mer peptides with residue overlaps of 14 residues were spotted on microarrays and incubated with serum mix from five tumor-bearing animals with high titers against both L1 isoforms. Bound serum antibodies were detected with fluorophore-conjugated secondary antibodies. Fig 5. PMID: 32746966.
Western Blot Results using Goat Anti-Mouse IgG (H&L) Antibody DyLight™ 680 Conjugated. Expression levels of Bcl-2, Bax, caspase-3 and caspase-9 proteins in the DRG. (A) The expression level of Bcl-2 protein in rat bladders was detected by western blot analysis. (B) The expression levels of Bax, caspase-3, cleaved caspase-3, caspase-9 and cleaved caspase-9 proteins in rat bladders were detected by western blot analysis. *P<0.05 vs. control group; #P<0.05 vs. caffeine group; @P<0.05 vs. DM group. Bcl-2, B-cell lymphoma-2; Bax, Bcl-2-associated X protein; DRG, dorsal root ganglion; DM, diabetes mellitus. Fig 3. PMID: 33791010.
Western Blot Results using Goat Anti-Mouse IgG (H&L) Antibody DyLight™ 680 Conjugated. TGF-B Beta-mediated crosstalk between pericytes and CRC cells modulates pericyte secretome. (A) Incubation in HCT116 CM for 1 h induces SMAD3 phosphorylation in PC, as assessed by western blot. Exogenous recombinant TGF-B Beta (10 ng mL-1) was used as a positive control, and B Beta-actin was used as loading control (n = 3). (B) Confocal microscopy images of SMAD3 subcellular localization in PC cultured alone or cocultured with HCT116 cells for 48 h (n = 3). SMAD3 is detected in the cytoplasm of PC in monoculture (arrows show nonstained nuclei). Nuclear translocation of SMAD3 takes place after coculture with HCT116 cells for 48 h (arrowheads indicate stained nuclei). HCT116 cells treated with 10 ng mL-1 TGF-B Beta1 were used as a positive control. Scale bar = 10 um. Fig 5. PMID: 32767843.
Western Blot of Goat anti-Mouse IgG Antibody DyLight 680 Conjugated Pre-absorbed. Lane 1: Mouse IgG. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Goat anti-Mouse IgG Antibody DyLight 680 Conjugated Pre-absorbed at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 25 kDa for Mouse IgG.
DyLight™ dyes can be used for two-color Western Blot detection with low background and high signal. Anti-tubulin was detected using a DyLight™ 680 conjugate. Anti-TNFa was detected using a DyLight™ 800 conjugate. The image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.