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Antibodies

Mouse IgG Fc Antibody DyLight[TM] 649 Conjugated

Product Sizes
100 ug
610-143-003-100UG
About this Product
SKU:
610-143-003
Additional Names:
Goat Anti Mouse IgG F(c) Antibody DyLight™ 649 Conjugated, Goat Anti-Mouse IgG Fc Antibody DyLight™ 649 Conjugated, Goat Anti Mouse IgG Fc Fragment Antibody DyLight™ 649 Conjugated
Application:
FLISA, Immunofluorescence, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
DyLight™ 649
Concentration:
1 mg/ml
Extra Details:
Anti-Mouse IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of mouse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Mouse IgG F(c) fragment
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Mouse
Shipping Conditions:
Ambient
Specificity:
IgG Fc|This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against Mouse IgG F(ab) This a
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
ERK is required for fibrous tissue marker regulation but not required to inhibit chondrogenesis. (A) Sclerotome was treated with a MEK inhibitor PD184352, PD, to inhibit ERK activity, for 24 h and then cells were treated with TGFB Beta1 for 8 h. Immunoblot was used to determine relative levels of pERK1/2, ERK1/2, and A Alpha-tubulin. Primary antibodies: pERK1/2 , ERK1/2, and Alpha tubulin [p/n 200-301-880] with secondary antibodies: anti-Rabbit-HRP and anti-mouse DyLight™649 [p/n 610-143-003]. Figure 5. PMID: 33288795.
TGF-B Beta signaling regulates noncanonical pathways in the sclerotome. Sclerotome was treated with vehicle control or TGFB Beta1 for 2, 8 or 24 h. Immunoblot was used to determine activity of ERK, p38 and AKT. A Alpha tubulin was used as a general loading control. Immunoblots were cropped for clarity. Primary antibodies: pERK1/2 , ERK1/2, pp38, p38, pAKT, AKT, and Alpha tubulin [p/n 200-301-880] with secondary antibodies: anti-Rabbit-HRP and anti-mouse DyLight™649 [p/n 610-143-003]. Figure 3. PMID: 33288795.
Knock-down of Sox9 attenuates TGF-B Beta-mediated regulation of Papss2 ATDC5 cells were transfected with Sox9 siRNA, Smad2/3 siRNA, or control non-specific (NS) siRNA. Western blots showed Smad2/3 protein levels were reduced in the presence of Smad2/3 siRNA, A Alpha-Tubulin was used as a loading control (n = 6) (D). Primary antibodies: anti-SMAD2/3 antibody (1:2000) or anti-A Alpha-Tubulin antibody [1:2500, p/n 200-301-880], with secondary antibodies anti-mouse [1:2500, p/n 610-143-003]. Figure 4. PMID: 27746378.
SMAD3 and SOX9 adenoviral vectors infect cells, induce expression of FLAG-tagged proteins, and up-regulate SMAD3 and SOX9 function respectively Bovine chondrocytes that were infected with either Ad-eGFP, Ad-SMAD3, or Ad-SOX9. Western blots of extracts from bovine chondrocytes showed expression of FLAG-tagged proteins at approximately 52 kDa and 75 kDa, corresponding to SMAD3 and SOX9 molecular weights respectively (n = 4) (B). Primary antibodies: anti-GAPDH antibody (1:1000) or anti-FLAG antibody (1:500), with secondary antibodies: HRP-conjugated anti-rabbit (1:2000) and anti-mouse [1:2500, p/n 610-143-003] Figure 2. PMID: 27746378.
DyLight™ dyes can be used for two-color Western Blot detection with low background and high signal. Anti-tubulin was detected using a DyLight™ 549 conjugate. Anti-TNFa was detected using a DyLight™ 649 conjugate. The image was captured using the Typhoon™ 9410 Imaging System.