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Antibodies

Mouse IgG (H&L) Antibody

Product Sizes
2 mg
610-1102-2MG
About this Product
SKU:
610-1102
Additional Names:
Goat anti-Mouse IgG Secondary Antibody, Mouse Secondary Antibody, GAM Antibody, Gt-a-Ms antibody, anti-mouse secondary, Goat anti Mouse IgG
Application:
ELISA, FLISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Lateral Flow Assay, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
2.19 mg/ml
Extra Details:
Anti-Mouse IgG Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This unconjugated secondary antibody has been validated and optimized yielding good sensitivity and reproducible results with Rockland's primary antibodies.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in goat.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Mouse
Shipping Conditions:
Blue Ice
Specificity:
IgG (H&L)|Secondary Antibody Anti-Mouse IgG (H&L) was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
ELISA Results of Goat Anti-Mouse IgG Antibody tested against purified Mouse IgG protein. Each well was coated in duplicate with 1.0 ug of Mouse IgG (p/n 010-0102). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. The titer is 1:35,800. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Donkey Anti-Goat IgG Peroxidase Conjugate at 1:40,000 and TMB substrate p/n TMBE-1000.
Western Blot of Goat Anti-Mouse IgG (H&L) Antibody. Lane M: Molecular Ladder. Lane 1: Mouse IgG whole molecule (p/n 010-0102). Lane 2: Mouse IgG F(c) Fragment (p/n 010-0103). Lane 3: Mouse IgG F(ab) Fragment (010-0105). Lane 4: Mouse IgM Kappa (p/n 010-001-339). Lane 5: Mouse Serum (p/n D308). Load: 50ng per lane. Block: (p/n MB-070) for 30 min at RT. Primary Antibody: Anti-Mouse IgG (H&L) Antibody 1:1000 for 60 min at RT. Secondary antibody: HRP Donkey Anti-Goat IgG (p/n 605-703-125) at 1:40,000 for 30 min at RT . Predicted/Observed Size: 28 and 55 kDa for Mouse IgG, F(c), F(ab), IgM Kappa, and Serum. Mouse F(c) migrates slightly higher.
Western Blot of Goat anti-Mouse IgG (H&L) Antibody. Lane 1: Mouse IgG. Load: 100 ng per lane. Primary Antibody: Mouse IgG (H&L) Antibody 1:1000 overnight at 4°C. Secondary antibody: DyLight™ 800 goat secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed Size: 28 and 55 kDa/28 and 55 kDa for Mouse IgG. Other band(s): none.