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Antibodies

Human IgM (mu chain) Antibody DyLight[TM] 549 Conjugated

Product Sizes
100 ug
609-142-007-100UG
About this Product
SKU:
609-142-007
Additional Names:
Goat Anti-Human IgM (mu chain) Antibody DyLight™ 549 Conjugated, Goat Anti Human IgM (mu chain) Antibody DyLight™ 549 Conjugated
Application:
FLISA, Immunofluorescence, Microarray, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
DyLight™ 549
Concentration:
1 mg/ml
Extra Details:
Anti-Human IgM (mu heavy chain) DyLight 549 generated in goat detects specifically Human IgM mu heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Anti-Human IgM mu chain antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Human IgM whole molecule
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Human
Shipping Conditions:
Ambient
Specificity:
IgM u chain|This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgM and Human Serum. No reaction was observed against other human heavy or light chain
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
Identification of four immunogenic MERS-CoV-S epitopes. (a) Schematic representation of the MERS-CoV-S protein. The N-terminal domain (NTD), receptor-binding domain (RBD), S1/S2 cleavage site (S1/S2), fusion peptide (FP), S2' cleavage site (S2'), heptad repeat 1 and 2 (HR1, HR2), transmembrane domain (TM) and cytoplasmic domain (CD) are illustrated. (b) Microarray of 15-mer peptides spanning the complete MERS-CoV-S protein with a 13 amino acid (AA) overlap. Immunogenic B-cell peptides are marked with red lines. (c-f) IgG binding to the respective peptides on MERS-CoV-S was measured in fluorescence intensity (as arbitrary fluorescence units, AFU), depicted as transformed values (areas sinus hyperbolicus (asinh), y axis), in all booster study participants (n = 10). Mean levels of peptide-binding IgG at baseline (Day (D) 0 (D0), white boxes) and 28 days after booster vaccination (Boost Day (B:D) 28 (B:D28), gray boxes) were compared using a two-sided Wilcoxon matched-pairs signed rank test and are depicted for each peptide (x-axis) within the immunogenic epitopes c AA 535-553 (AA 535-549, p = 0.014; AA 537-551, p = 0.002; AA 539-553, p = 0.002), d AA 887-913 (AA 887-901, p = 0.027; AA 889-903, p = 0.006; AA 891-905, p = 0.002; AA 897-911, p = 0.002; AA 899-913, p = 0.002), e AA 1225-1247 (AA 1225-1239, p = 0.002; AA 1227-1241, p = 0.002; AA 1229-1243, p = 0.002; AA 1231-1245, p = 0.002; AA 1233-1247, p = 0.004) and f AA 1333-1353 (AA 1333-1347, p = 0.002; AA 1335-1349, p = 0.002; AA 1337-1351, p = 0.002; AA 1339-1353, p = 0.002). Boxes indicate 25-75 percentile; whiskers are min. to max.; medians are shown as horizonal lines within the boxes. *p < 0.05, **p < 0.005. Source data are provided as a Source Data file. Fig. 5. PMID: 35853863.
Western Blot analysis of IgG and IgM antibodies against R. helvetica whole cell antigen demonstrates the lipopolysaccaride (LPS) ladders and specific reactions against R. helvetica proteins in the 110-150-kDa region in serum for IgG for patients 1, 32 and 33 and for IgM for patients 3, 8, 10, 17, 20 and 22 in dilution 1:200. Lane P(h) demonstrates specific proteins and the LPS ladders reacting with a positive human serum and P(r) with a polyclonal rabbit antiserum. N(h) represent a negative human serum control. Secondary antibody Anti-human IgG DyLight™549 (p/n 609-142-123 ) and Anti-human IgM DyLight™ 549 (p/n 609-142-007). Figure 1. PMID: 34712390.
DyLight™ dyes can be used for two-color Western Blot detection with low background and high signal. Anti-tubulin was detected using a DyLight™ 549 conjugate. Anti-TNFa was detected using a DyLight™ 649 conjugate. The image was captured using the Typhoon™ 9410 Imaging System.
Properties of DyLight™ Conjugates.