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Antibodies

Goat IgG (H&L) Antibody DyLight[TM] 488 Conjugated

Product Sizes
100 ug
£177.00
605-741-002-100UG
About this Product
SKU:
605-741-002
Additional Names:
donkey anti-Goat IgG Antibody DyLight™ 488 Conjugation, donkey anti-Goat IgG DyLight™ 488 Conjugated Antibody
Application:
Dot Blot, FLISA, Immunofluorescence, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
DyLight™ 488
Concentration:
1 mg/ml
Extra Details:
Anti-Goat IgG DyLight Antibody generated in donkey detects goat IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Donkey
Immunogen:
Goat IgG, whole molecule
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Goat
Shipping Conditions:
Ambient
Specificity:
IgG (H&L)|This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Goat IgG and Goat Serum. This antibody will react with heavy chains of Go
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles., -20[o]C/-70[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
FluoroFinder:
Maternal exposure to Printex 90 carbon nanoparticles dose-dependently increases expression levels of glial fibrillary acidic protein (GFAP) in astrocytes in the offspring cerebral cortex. a, b, d, e, g and h show representative fluorescent micrographs of GFAP-positive astrocytes in the cerebral cortices of 6 week old male offspring in the control (a, b), low exposure (d, e), and high exposure groups (g, h). a, d and g gives an overview of the cerebral cortex, with b, e, and h providing enlarged views hereof, respectively. c, f, and i are grayscale views of b, e, and h, respectively, for quantification of the GFAP expression. Fig 4. PMID: 30201004.
Maternal exposure to Printex 90 carbon nanoparticles dose-dependently increases expression levels of glial fibrillary acidic protein (GFAP) in astrocytes in the offspring hippocampus. a, b, d, e, g and h show representative fluorescent micrographs of GFAP-positive astrocytes in the hippocampi of 6 week old male offspring in the control (a, b), low exposure (d, e), and high exposure group (g, h). a, d and g gives an overview of the hippocampus, with b, e, and h providing enlarged views of panels a, d, and g, respectively. c, f, and i are grayscale views of b, e, and h, respectively, for quantification of GFAP expression. Fig. 5. PMID: 30201004.
DyLight™ dyes can be used for multi-color immunofluorescence microscopy with uniform fluorescence intensity throughout the image. DyLight™ dyes are exceptionally bright and photostable and are optimized for microscopy and microarray detection methods. This image shows anti-histone detection using a DyLight™ 488 conjugate (green). Anti-Tubulin was detected using a DyLight™ 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc).
DyLight™ 488 Fluorescence Spectra.
Properties of Dylight™ Conjugates.