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Antibodies

Goat IgG (H&L) Antibody ATTO 647N Conjugated Pre-Adsorbed

Product Sizes
500 ug
605-456-013-500UG
About this Product
SKU:
605-456-013
Additional Names:
rabbit anti-Goat IgG ATTO 647N Conjugated Antibody, rabbit anti-Goat IgG Antibody ATTO647N Conjugation
Application:
Dot Blot, FLISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
ATTO 647N
Concentration:
1 mg/ml
Extra Details:
Anti-Goat IgG ATTO dye Antibody generated in rabbit detects goat IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Goat IgG whole molecule
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Goat
Shipping Conditions:
Ambient
Specificity:
Goat IgG (H&L) Antibody ATTO 647N was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against Human, Mouse or Rabbit Se
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
ATTO ® dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Joerg Reichwein, ATTO-TEC GmbH
Dot Blot Results of Rabbit Anti-Goat IgG (H&L) Antibody (MX Hu, Ms, Rb) ATTO 647N Conjugated. Sample: Goat IgG [p/n 005-0102]. Loaded: 1) 100ng, 2) 33.33ng, 3) 11.11ng, 4) 3.7ng, 5) 1.23ng. Antibody: Anti-Goat IgG (H&L) [Rabbit] Antibody (MX3) ATTO 647N Conjugated at 1.0ug/mL for 1hr at RT. Block: Blocking Buffer for Fluorescent Western Blotting [p/n MB-070] for 30 mins at RT.
Comparison of nephrin imaging with different conventional and super-resolution modalities. A) Widefield vs dSTORM using Atto647N. B) Confocal vs STED using Atto647N. C) Widefield vs SIM using Alexa Fluor 488. D) ExM prepared sample imaged with widefield vs e confocal using Alexa Fluor 488. The confocal ExM image furthermore shows the nucleus stained with Hoechst. Highlighted panels show x 3 zoomed areas. Scale bars in a-c are 1 um, in d and e 10 um and in the zoomed in areas 5 um (after expansion) Figure provided by CiteAb. Source: Anal Bioanal Chem, PMID: 33277998.