Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Goat IgG (H&L) Antibody ATTO 425 Conjugated Pre-Adsorbed

Product Sizes
500 ug
£400.00
605-451-013-500UG
About this Product
SKU:
605-451-013
Additional Names:
rabbit anti-Goat IgG ATTO 425 Conjugated Antibody, rabbit anti-Goat IgG Antibody ATTO425 Conjugation
Application:
FLISA, Immunofluorescence, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
ATTO 425
Concentration:
1 mg/ml
Extra Details:
Anti-Goat IgG ATTO dye Antibody generated in rabbit detects goat IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Goat IgG whole molecule
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Goat
Shipping Conditions:
Ambient
Specificity:
Goat IgG (H&L) Antibody conjugated to ATTO425 was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against Human, Mouse
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles., -20[o]C/-70[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
FluoroFinder:
ATTO ® dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Joerg Reichwein, ATTO-TEC GmbH
ATTO 425 conjugated anti-Mouse IgG was used to demonstrate 2 color STED immunofluorescence microscopy. Methanol fixed A431 cells were blocked with normal goat serum. The cells were then probed with 0.4 ug/mL final concentration of anti-a-tubulin and detected with 0.2 ug/mL ATTO 425 conjugated anti-MOUSE IgG [GOAT] (610-151-121) secondary antibody (colored RED). Also shown in this 2-color STED image is Rockland's Anti-HDAC-1 [RABBIT] (p/n 600-401-879) detected with DyLight™488 conjugated Anti-RABBIT IgG [GOAT] secondary antibody (colored GREEN). Image courtesy of Myriam Gastard, Leica Microsystems, USA.