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Antibodies

Chicken IgG (H&L) Antibody

Product Sizes
10 mg
603-4102-10MG
About this Product
SKU:
603-4102
Additional Names:
Rabbit anti-Chicken IgG Antibody, Chicken Secondary Antibody, Rabbit anti-Chicken IgY
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
10.14 mg/ml
Extra Details:
Anti-Chicken IgG Antibody generated in rabbit detects chicken IgY. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-Chicken IgG (H&L) was produced by repeated immunization with Chicken IgG whole molecule in rabbit.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Avian
Shipping Conditions:
Blue Ice
Specificity:
IgG (H&L)|This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Chicken IgG. Some light chain cross reactivity may be observed against chicken immunoglobulins.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
Western Blot of Unconjugated Anti-Chicken IgG (H&L) (RABBIT) Antibody (p/n 603-4102). Lane M: 3 ul Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG Fab Fragment (p/n 003-0105). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG (H&L) (RABBIT) Antibody (p/n 603-4102) 1:3,000 for 60 min at RT. Secondary antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n 611-103-122) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY, 25 kDa for F(c) and Fab. Chicken F(c) migrates slightly higher.
Western Blot of Rabbit anti-Chicken IgG Antibody. Lane 1: Chicken IgG (p/n 003-0102). Load: 100 ng per lane. Primary antibody: Chicken IgG antibody at 1:1000 for overnight at 4°C. Secondary antibody: DyLight™ 649 rabbit anti-Chicken secondary antibody (p/n 603-443-002) at 1:20,000 for 30 min at RT. Block: 5% BLOTTO (B501) for 30 min at RT. Predicted/Observed size: 72 and 28 kDa, 72 and 28 kDa for Chicken IgY.