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Antibodies

Chicken IgG Fc Antibody Rhodamine Conjugated

Product Sizes
1.5 mg
£195.00
603-4003-1.5MG
About this Product
SKU:
603-4003
Additional Names:
rabbit anti-Chicken IgG F(c) Antibody Rhodamine Conjugation, rabbit anti-Chicken IgG F(c) TRITC Conjugated Antibody, rabbit anti-Chicken IgY F(c) Antibody Rhodamine Conjugation, rabbit anti-Chicken IgY F(c) TRITC Conjugated Antibody
Application:
FLISA, Flow Cytometry, Immunofluorescence
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
TRITC
Concentration:
1.5 mg/ml
Extra Details:
Anti-Chicken IgG F(c) Antibody detects specifically Chicken IgY F(c). It is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of chicken IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Anti-Chicken IgG F(c) Antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Chicken IgG F(c) fragment
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Avian
Shipping Conditions:
Ambient
Specificity:
IgG Fc|This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Chicken IgG, Chicken IgG F(c) and Chicken Serum. No reaction was observed against Chicken IgG
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles., -20[o]C/-70[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
FluoroFinder:
Western Blot of Unconjugated Anti-Chicken IgG F(c) (RABBIT) Antibody (p/n 603-4103). Lane M: 3 ul Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG Fab Fragment (p/n 003-0105). Lane 4: Chicken IgM Whole Molecule (p/n 003-0107). Lane 5: Chicken Serum (p/n D302-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG F(c) (RABBIT) Antibody (p/n 603-4103) 1:2,000 for 60 min at RT. Secondary antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n 611-103-122) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY and Serum, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher.