6X His Tag Antibody Dylight[TM] 549 Conjugated
Product Sizes
100 ug
600-442-382-100UG
About this Product
- SKU:
- 600-442-382
- Additional Names:
- rabbit anti-6X HIS EPITOPE TAG Antibody DyLight™549 conjugation, DyLight™549 conjugated rabbit anti-6X HIS EPITOPE TAG Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody
- Application:
- ELISA, FLISA, Immunofluorescence, Western Blot
- Buffer:
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
- CE/IVD:
- RUO
- Clonality:
- Polyclonal
- Conjugate:
- DyLight™ 549
- Concentration:
- 1 mg/ml
- Extra Details:
- Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Rockland Immunochemicals also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.
- Formulation:
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Host:
- Rabbit
- Immunogen:
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the 6X HIS epitope tag (H-H-H-H-H-H) conjugated to KLH using maleimide.
- Isotype:
- IgG
- Physical State:
- Lyophilized
- Reactivities:
- Label/Epitope-tag
- Shipping Conditions:
- Ambient
- Specificity:
- This affinity purified antibody is directed against the 6X HIS epitope tag and is useful in determining its presence in over expressed proteins in various assays. The antibody recognizes the 6X HIS epitope tag (His-His-His-His-His-His) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
- Storage Conditions:
- 2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Rockland Inc
- Type:
- Antibody: Tagged Antibodies
- Manufacturer's Data Sheet:https://www.rockland.com/search/?searchString=600-442-382
