Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

AES Antibody

Product Sizes
100 ug
£683.00
600-401-X76-100UG
About this Product
SKU:
600-401-X76
Additional Names:
AES|AES Antibody, GRG, ESP1, GRG5, TLE5, AES-1, AES-2, GRG, Amino-terminal enhancer of split, Gp130-associated protein GAM, Amino enhancer of split
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apoptosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-AES antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid synthetic peptide from near the amino-terminus of human AES.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Specificity:
Anti-AES Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with AES from other sources has not been determined.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of AES antibody. Lane A: 293 cell lysate with AES antibody at 2 ug/mL. Lane B: 293 cell lysate with AES antibody at 4 ug/mL. Load: 35 ug per lane. Primary antibody: AES antibody at designated concentrations for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 22 kDa, 20 kDa for AES. Other band(s): AES splice variants and isoforms.
Immunocytochemistry of AES antibody. Cell Type: 293 cells. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: AES antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: AES is nuclear. Staining: AES is stained brown with hematoxylin purple counterstain.
Immunofluorescence Microscopy of AES antibody. Cell Type: 293 cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: AES antibody at 20 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: AES is nuclear. Staining: AES as red fluorescent signal.