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Antibodies

Glucagon Antibody

Product Sizes
100 ug
£600.00
600-401-MV9-100UG
About this Product
SKU:
600-401-MV9
Additional Names:
GCG|Rabbit Anti-Glucagon, Pro-glucagon, Glicentin, Oxyntomodulin, OXM, OXY, Glucagon
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1.18 mg/ml
Extra Details:
Glucagon is a member of a multigene family that includes secretin. Glucagon is a 29-amino acid pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. The human glucagon gene is approximately 9.4 kb long, contains 6 exons and 5 introns, and assigned to 2q36-2q37. This antibody is suitable for researchers interested in metabolic diseases, like diabetes, cell proliferation, differentiation, apoptosis, GCPR signaling, and calcium signaling research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Glucagon antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human Glucagon.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
This affinity purified antibody is directed against human Glucagon. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to human, chimpanzee, and bonobo.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
ELISA Results of Rabbit Anti-Glucagon Antibody. Each well was coated with 1ug of conjugate. The starting concentration of antibody in the dilution series was 5 ug/ml. The titer is 1:34,800 Glucagon - Free peptide [Green Line], 1:47200 Glucagon Standard - BSA conjugated [Blue Line], and 1:20,500 Glucagon - BSA conjugated [Purple Line]. Each point on the Y-axis represents a 3-fold dilution. 3% Fish Gel (p/n MB-066-0100), HRP conjugated Goat anti-Rabbit IgG (H&L) (p/n 611-1302), and TMB substrate (p/n TMB-1000) were used for detection.
Immunofluorescence of Rabbit Anti-Glucagon Antibody. Cell Line: NIH/3T3 cells. Fixative: 100% Methanol. Permeabilization: Triton X-100. Primary Antibody: Anti-Glucagon at 15ug/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG DyLight™488 (p/n 611-141-122) at 5uL/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A). DAPI. (B). Anti-Glucagon + DyLight™488 secondary. (C). Merge A+B. (D). secondary only. Localization expected: Cytoplasm.
Immunohistochemistry results using Rabbit Anti-Glucagon Antibody. Tissue: alpha cells in CD1 mouse pancreatic islets. Fixation: 4% paraformaldehyde. Antigen Retrieval: 10mM Sodium Citrate buffer for 10 mins at 95-100°C. Blocking: PBS, 1% ovalbumin, 0.3% Triton X-100. Primary Antibody: Anti-Glucagon at 1:100 overnight at RT. Secondary Antibody: Anti-Rabbit Alexa Fluor 488 at 1:500 for 1hr at RT. Original magnification 20x. Independently Validated by antibodies-online GmbH (ABIN7448121). Courtesy of Prof. Merighi, University of Turin.
Immunofluorescence of Rabbit Anti-Glucagon Antibody. Cell Line: NIH/3T3 cells. Fixative: 4% PFA. Permeabilization: Triton X-100. Primary Antibody: Anti-Glucagon at 15ug/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG DyLight™488 (p/n 611-141-122) at 5uL/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A). DAPI. (B). Anti-Glucagon + DyLight™488 secondary. (C). Merge A+B. (D). secondary only. Localization expected: Cytoplasm.
Western Blot of Rabbit Anti-Glucagon Antibody. Lane 1: Opal Prestained Molecular Weight (p/n MB-210-0500). Lane 2: COS-7 Lysate - reduced (20ug). Lane 3: BSA Conjugated Glucagon peptide - reduced (0.02ug). Lane 4: Insulin - reduced (0.05ug). Primary Antibody: Anti-Glucagon [Rabbit] Antibody at 1.0ug/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG (MX10) Peroxidase conjugated at 1:70,000 for 30mins at RT. Block: Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) for 1hr at RT. Expected MW: ~21kDa. Observed MW: endogenous detection in COS-7 Lysate at ~35kDa. Glucagon peptide is detected at the MW of BSA. No cross-reactivity with insulin is observed. Exposure: 25 sec.