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Antibodies

CREBBP Antibody

Product Sizes
25 ul
600-401-MQ0S-25UL
About this Product
SKU:
600-401-MQ0S
Additional Names:
CREBBP|Rabbit Anti-CREBBP Antibody, Rabbit Anti-CREB Binding Protein, CREB-Binding Protein, EC 2.3.1.48, CBP, Rubinstein-Taybi Syndrome, KAT3A, MKHK1, RSTS1, RSTS
Application:
ELISA, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
CREBBP (CREB Binding Protein) is ubiquitously expressed. It acetylates histones, giving a specific tag for transcriptional activation and acetylates non-histone proteins, like NCOA3 and FOXO1. It binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes, so it's known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. CREBBP acts as a coactivator of ALX1 and acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers. It acetylates PCNA, in which this acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER). Functions as a transcriptional coactivator for SMAD4 in the TGF-beta signaling pathway. This protein shares regions of very high sequence similarity with protein p300 in its bromodomain, cysteine-histidine-rich regions, and histone acetyltransferase domain. Bromodomains (BRDs) are epigenetic reader domains that selectively recognize acetylated lysine residues on the tails of histone proteins and are the only known protein modules that can target acetylated lysine residues. Mutations in this gene cause Rubinstein-Taybi syndrome (RTS) and acute myeloid leukemia. Anti-CREBBP Antibody is useful for researchers interested in Transcription Factors, Histones, Epigenetics, and Cancer research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-CREBBP antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an N-Terminal portion of human CREBBP conjugated to Keyhole Limpet Hemocyanin (KLH).
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Specificity:
This affinity purified antibody is directed against human CREBBP. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to mouse and rat.
Storage Conditions:
-20[o]C diluted. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
ELISA results of purified polyclonal Rabbit Anti-CREBBP Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Coating buffer, Goat Anti-Rabbit HRP conjugated (p/n 611-103-122) and substrate (p/n TMBE-1000).
Western Blot of Rabbit Anti-CREBBP Antibody. Lane 1: Opal Pre-Stained Marker (p/n MB-210-0500). Lane 2: Mouse Brain Whole Tissue Lysate (p/n W10-000-T004) [+]. Lane 3: Human Eye Tissue Lysate [-]. Load: 35ug lysate/lane on a 7.5% gel. Primary Antibody: Anti-CREBBP at 5ug/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:70,000 at RT. Block: Universal Buffer BlockOut (p/n MB-073). Predicted MW: ~265, 494kDa (Mouse), ~265kDa (Human). Observed MW: ~260, 270kDa. Exposure: 60sec.
Western Blot of Rabbit Anti-CREBBP Antibody. Lane 1: Opal Pre-Stained Marker (p/n MB-210-0500). Lane 2: MOLT-4 Whole Cell Lysate (p/n W09-001-GK2). Lane 3: HEK293T Whole Cell Lysate (p/n W09-001-GX5). Lane 4: A549 Whole Cell Lysate (p/n W09-001-372). Lane 5: PC-3 Whole Cell Lysate (p/n W09-001-GV6). Lane 6: Mouse Brain Whole Tissue Lysate (p/n W10-000-T004) [+]. Load: 35ug lysate/lane on a 7.5% gel. Primary Antibody: Anti-CREBBP at 5ug/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:70,000 at RT. Block: Universal Buffer BlockOut (p/n MB-073). Predicted MW: ~265, 494kDa (Mouse), ~265kDa (Human). Observed MW: >245kDa. Exposure: 30sec.