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Antibodies

VAChT Antibody

Product Sizes
25 ul
600-401-MK7S-25UL
About this Product
SKU:
600-401-MK7S
Additional Names:
Rabbit Anti-Vesicular acetylcholine transporter Antibody, VAChT, rVAT, solute carrier family 18 member 3, Slc18a3, Vacht, Vat|Slc18a3
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
0.90 mg/ml
Extra Details:
Slc18a3 (Solute Carrier Family 18 Member A3) is a Protein Coding gene. Vesicular acetylcholine transporters (VAChTs) are members of the solute carrier family 18 (SLC18) of ATP-dependent transporters that also includes vesicular monoamine transporters (VMAT) 1 and VMAT2. VAChT is found in the central and peripheral nervous systems. Transports acetylcholine into secretory vesicles for release into the extracellular space. Anti-VAChT Antibody is useful for researchers interested in Myasthenic syndromes, neurotransmitter release cycles, synaptic vesicle cycle, and neuroscience research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-VAChT antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of rat vesicular acetylcholine transporter conjugated to Keyhole Limpet Hemocyanin (KLH).
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Rat
Shipping Conditions:
Dry Ice
Specificity:
This affinity purified antibody is directed against rat VAChT. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to mouse and human.
Storage Conditions:
-20[o]C diluted. Avoid freeze/thaw cycles., See Manual Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunofluorescence of Rabbit Anti-VAChT Antibody. Cell Line: Post-natal rat pup (PND1) heterogeneous brain cells. Primary Antibody: Rabbit Anti-VAChT Antibody at 15ug/mL overnight at 4°C. Counterstain Antibody: Donkey Anti-Rabbit IgG DyLight™ 488 Conjugated (p/n 611-741-127) at 5ug/mL for 1hr at RT. Fixative: 4% PFA. Permeabilization: 0.3% Triton X-100. Nuclear Counter Stain: DAPI. Image: A) DAPI (blue). B) Anti-VAChT (green) and Secondary. C) Merge of A+B images. D) Secondary only. Localization: predicted membrane, Golgi apparatus. Antibody detecting dendrites and axons and specific organelles in the nucleus (nucleolus). Staining correspondent to confirmed Slc18a3 staining in mouse brain P7 Gensat project: http://www.gensat.org/imagenavigator.jsp?imageID=66611
Immunohistochemistry of Rabbit Anti-VAChT Antibody. Tissue: Rat Spinal Cord. Primary Antibody: Rabbit Anti-VAChT Antibody at 1:100 for 30min at RT. Secondary Antibody: Anti-Rabbit Poly-HRP-IgG. Ready to use 8 min at RT. Antigen Retrieval: HIER using Citrate Buffer for 20 min. Counterstain: Hematoxylin.
Western Blot of Rabbit Anti-VAChT Antibody. Molecular Weight Marker: Opal Pre-stained (p/n MB-210-0500). Lane 1: Rat brain, post natal, Whole Cell Lysate (p/n W12-001-MQ6). Primary Antibody: Anti-VAChT Antibody at 1:1000 overnight at 4°C. Secondary Antibody: Donkey Anti-Rabbit IgG HRP (p/n 611-703-127) at 1:70,000 for 1hr at RT. Blocking: BlockOut Universal Buffer (p/n MB-073). Expected: ~57 kDa, protein glycosylation.