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Antibodies

Beclin 1 Antibody

Product Sizes
100 ug
£600.00
600-401-MG4-100UG
About this Product
SKU:
600-401-MG4
Additional Names:
BECN1|Rabbit Anti-Beclin-1, Beclin1 Antibody, Beclin 1 Antibody, BECN1, Coiled-Coil Myosin-Like BCL2-Interacting Protein, Beclin 1 Autophagy Related, Beclin 1 (Coiled-Coil, Moesin-Like BCL2 Interacting Protein), ATG6 Autophagy Related 6 Homolog, Protein GT197, VPS30, GT197
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
BECLIN1 belongs to the beclin family. Beclin 1 plays a central role in autophagy, a catabolic process of degradation induced by starvation. Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus. Localized in the Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells. Anti-Beclin-1 Antibody is useful for researchers interested in Cancer Research including Cervix Carcinoma and Breast Cancer.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-BECLIN1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-terminal portion of human Beclin-1 conjugated to Keyhole Limpet Hemocyanin (KLH).
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Specificity:
This affinity purified antibody is directed against human Beclin-1. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to human, mouse, rat, bovine, pongo abelii, and wild boar and 92% to Gallus gallus.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunofluorescence of Rabbit Anti-Beclin 1 Antibody. Cells: HeLa cells. Fixative: 100% MeOH. Permeabilizaton: 0.3% Triton X-100. Primary Antibody: Anti-Beclin-1 at 5ug/mL overnight at 2-8°C. Secondary Antibody: Donkey Anti-Rabbit IgG DyLight™488 (p/n 611-741-127) at 5ug/mL for 60mins at RT. Nuclear Counterstain: DAPI. Staining: (A) DAPI, (B) Anti-Beclin1+DyLight, (C) Merge A+B, (D) secondary only. Expected localization:: Golgi apparatus, mitochondria, endosome, nucleus.
Immunohistochemistry of Rabbit Anti-Beclin 1 Antibody. Tissue: Human Spleen. Fixative: none. Antigen Retrieval: HIER using Citrate buffer for 20 minutes. Primary Antibody: Anti-Beclin 1 at 1:100 for 30mins at RT. Secondary Antibody: Anti-Rabbit Poly-HRP-IgG ready to use 8 min at RT. Counterstain: hematoxylin. Expected Localization: nuclear or cytoplasmic staining of normal cells. Less staining of cancer cells. Result Analysis: shows diffuse moderate cytoplasmic/membranous staining of cells in the white and red pulp in human splenic tissue. Cell staining includes lymphocytes and macrophages as well as endothelial cells.
Western Blot of Rabbit Anti-Beclin 1 Antibody. Lane 1: Opal Prestain Molecular Weight Marker (p/n MB-210-0500). Lane 2: Beclin overexpressing HEK293T lysate (10ug) [+]. Lane 3: HEK293T vector vector (10ug) [-] (p/n W09-001-GX5). Lane 4: U87-MG whole cell lysate (35ug) [+]. Lane 5: C2C12 whole cell lysate (35ug) [+] (p/n W10-001-GL7). Lane 6: MOLT4 whole cell lysate (35ug) [+] (p/n W09-001-GL2). Lane 7: U251-MG whole cell lysate (35ug) [+] (p/n W09-001-GY4). Lane 8: Opal Prestain Molecular Weight Marker (p/n MB-210-0500). Primary Antibody: Anti-Beclin1 at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:40,000 for 60min at RT. Block: 5% BLOTTO/TBST (p/n B501-0500). Expected MW: ~51kDA endogenous, ~50-60kDa overexpressing. Observed MW: ~53, 75kDa.
Immunohistochemistry of Rabbit Anti-Beclin 1 Antibody. Tissue: Human colon cancer tissue (40X). Fixative: none. Antigen Retrieval: Heat induced epitope retrieval (HIER). Primary Antibody: Anti-Beclin 1 at 1:100 for 30mins at RT. Secondary Antibody: Anti-Rabbit Poly-HRP-IgG ready to use 8 min at RT. Counterstain: hematoxylin. Expected Localization: nuclear or cytoplasmic staining of normal cells. Less staining of cancer cells. Result Analysis: Passed. Epithelial layer staining, nuclear staining, cell-cell junction staining.