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Antibodies

Beta Amyloid pyro E3 Antibody

Product Sizes
50 ug
600-401-J14-50UG
About this Product
SKU:
600-401-J14
Additional Names:
APP|rabbit anti-Beta Amyloid pyro E3 Antibody, B Beta-amyloid pyro E3, Alzheimer disease amyloid protein, Beta amyloid, A-beta, ABPP, APPI
Application:
Detection/Quantitation, Dot Blot, ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
The cerebral and vascular plaques associated with Alzheimer's disease are mainly composed of Amyloid beta peptides. Beta Amyloid is derived from cleavage of the Amyloid precursor protein and varies in length from 39 to 43 amino acids. Beta Amyloid [1-40], beta Amyloid [1-42], and beta Amyloid [1-43] peptides result from cleavage of Amyloid precursor protein after residues 40, 42, and 43, respectively. The cleavage takes place by gamma-secretase during the last Amyloid precursor protein processing step. Beta Amyloid [1-40], beta Amyloid [1-42], and beta Amyloid [1-43] peptides are major constituents of the plaques and tangles that occur in Alzheimer's disease. Beta Amyloid antibodies and peptides have been developed as tools for elucidating the biology of Alzheimer's disease. Anti-beta Amyloid [Pyro Glu11] Antibody is ideal for researchers interested in Alzheimer's Research, Cell Cycle and Replication, and Neuroscience research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Beta Amyloid [Pyro Glu3] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-terminus 3-pyro E start point of human beta Amyloid.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
Anti-Beta Amyloid pyro Glu3 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody contains no reactivity towards the 1-42 ABeta peptide. A BLAST analysis was used to suggest cross-reactivity with Human, Primate, Bovine and Sheep based on 100% sequence homology. It is not reactive with Rodentia. Cross-reactivity with beta Amyloid pyro Glu3 from other sour
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunofluorescence Microscopy of Rabbit Anti-beta Amyloid pyro E3 antibody. Tissue: human brain section. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: beta Amyloid pyro E3 antibody at 5 ug/mL for 1 h at RT. Secondary antibody: Rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: beta Amyloid pyro E3 is nuclear and cytoplasmic. Staining: Top: B Beta Amyloid pyro E3 as green fluorescent signal, B Beta Amyloid 3 as yellow signal; and Bottom: B Beta Amyloid 3 as red signal with co-incubation of B Beta Amyloid pyro E3 peptide.
Western Blot of Rabbit Anti-beta Amyloid pyro E3 antibody. Lane 1: beta Amyloid 3 peptide Lane 2: beta Amyloid pyro E3 peptide. Load: 1 ug per lane. Primary antibody: B Beta Amyloid pyro E3 antibody at 2ug/mL for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 86.9 kDa for beta Amyloid pyro E3 peptide.
Western Blot of Rabbit Anti-beta Amyloid pyro E3 antibody. Lane 1-10: beta Amyloid pyro E3 peptide. Load: 1 ug per lane 1-2, 0.2 ug per lane 3-4, 0.05 ug per lane 5-6, 0.01 ug per lane 7-8, 0.005 ug per lane 9-10. Primary antibody: B Beta Amyloid pyro E3 antibody at 0.5 ug/mL for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 86.9 kDa for beta Amyloid pyro E3 peptide. Cross reactivity with B Beta Amyloid 3 peptide is >1000 fold below pyrE3 reactivity and contains no reactivity towards the B Beta Amyloid 42 AB Beta peptide (data not shown).