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Antibodies

ZO-1 Antibody

Product Sizes
100 ug
£600.00
600-401-GU7-100UG
About this Product
SKU:
600-401-GU7
Additional Names:
rabbit anti-ZO-1 antibody, ZO 1, ZO1, Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1, TJP1|TJP1
Application:
Detection/Quantitation, ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1.1 mg/ml
Extra Details:
ZO-1, also called TJP1, belongs to the MAGUK family. This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. ZO-1 plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. ZO1 may be associated the following disorders; celiac disease, congenital nephrotic syndrome finnish type, and macular degeneration. Anti-ZO-1 Antibody is useful for researchers interested in Apoptosis Research and Insulin Research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-ZO-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human ZO-1 conjugated to Keyhole Limpet Hemocyanin (KLH).
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Specificity:
This affinity purified antibody is directed against human ZO-1. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with human.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunohistochemistry of Rabbit Anti-ZO-1 antibody. Tissue: mouse adipose tissue and muscle. Fixation: formalin fixed paraffin embedded. Antigen retrieval: heat induced (HIER) using Citrate Buffer for 20min. Primary antibody: ZO-1 antibody at 1:100 for 30min at RT. Secondary Antibody: Anti-Rabbit Poly-HRP-IgG Ready-to-Use for 8min at RT. Localization: ZO-1 will stain cell-cell junctions. Staining: DAB. Counter Stain: Hematoxylin.
Immunohistochemistry of Rabbit anti-ZO-1 antibody. Tissue: mouse adipose tissue. Fixation: formalin fixed paraffin embedded. Epitope retrieval: heat induced (HIER). Primary antibody: ZO-1 antibody at 1:100 [A] and 1:200 [B] for 1 h at RT. Localization: ZO-1 will stain cell-cell junctions. Visualized with WARP RED on MACH 4 universal AP polymer detection system.
Immunofluorescence Microscopy of Rabbit anti-ZO-1 antibody. Tissue: Caco2. Fixation: 0.5% PFA [A,C]. 0.5% MeOH [B,D]. Antigen retrieval: not required. Primary antibody: ZO-1 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Anti-RABBIT IgG DyLight™ 488 Conjugated Preadsorbed (p/n 611-741-127) at 5 ug/ml for 1 h at RT. Localization: (1) most epithelial cell junctions; (2) both in endothelial cells and the highly specialized epithelial junctions of renal and Sertoli cells. Staining: Target as green fluorescent signal with DAPI (blue) nuclear counterstain.
Immunofluorescence Microscopy of Rabbit anti-ZO-1 antibody. Tissue: CaCO2. Fixation: 4% PFA. Permeabilization: 0.3%Triton X-100. Primary antibody: ZO-1 antibody at 15ug/mL overnight at 2-8°C. Secondary antibody: Donkey Anti-Rabbit IgG DyLight™ 488 Conjugated Preadsorbed (p/n 611-741-127) at 5ug/mL for 1 h at RT. Localization: membrane. Staining: (A)DAPI. (B)ZO-1+DyLight488. (C)Merge A-B. (D) Secondary Only.
Immunofluorescence of ZO-1 and CD45. Representative confocal images of irradiated group (A) and controls (B) demonstrate a dramatic reduction of green fluorescence for ZO-1 in urothelium layer of irradiated group. Bladder sections were labeled with anti-ZO-1 antibodies and secondary donkey antibody Alexa 488 to localize the tight junction protein ZO-1 and with rhodamine-phalloidin to label the actin cytoskeleton (red) and DAPI to label the nuclei. Decreased expression of ZO-1 in urothelium layer of irradiated group was associated with an increase in CD45-positive, green fluorescent leukocytes (indicated by white arrows) in urothelium and lamina propria (C) compared with controls (D). Increased number of CD45-positive leukocytes indicates infiltration in irradiated bladder beyond the resident CD45 leukocytes spotted at one place in control section. Scale bar indicates magnification in different panels. Image Courtesy of Nishant Singh, Department of Urology, University of Pittsburgh, Pennsylvania.