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Antibodies

beta Catenin Antibody

Product Sizes
25 ul
600-401-C68S-25UL
About this Product
SKU:
600-401-C68S
Additional Names:
ctnnb1|rabbit anti-beta Catenin Antibody, rabbit anti-Catenin beta antibody, catenin beta-1, B Beta-catenin-1, B Beta-catenin, CTNNB1, CTNNB, beta-catenin antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1.15 mg/ml
Extra Details:
Beta-catenin 1 (or B Beta-catenin 1) is a protein that is encoded by the CTNNB1 gene. B Beta-catenin 1 is a subunit of the cadherin protein complex and has been implicated as an integral component in the Wnt signaling pathway. This pathway plays a key role in the regulation of cellular processes involved in development, differentiation, and adult tissue homeostasis. In the presence of Wnt ligand, B Beta-catenin 1 is not ubiquitinated and accumulates in the nucleus, where it associates with T-cell factor (TCF) family members to regulate target gene expression in many developmental and adult tissues. Recruitment of b-catenin 1 to Wnt response element (WRE) chromatin converts TCFs from transcriptional repressors to activators. B Beta-catenin 1 is also involved in the regulation of cell adhesion. It acts as a negative regulator of centrosome cohesion. Aberrant Wnt/B Beta-catenin signaling is widely implicated in cancer, bone disorders, kidney and intestinal cell disorders and other disease states. B Beta-catenin 1 is located in the cytoplasm when it is unstabilized or bound to CDH1. Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization. The majority of B Beta-catenin 1 is localized to the cell membrane. In interphase, colocalizes with CROCC between CEP250 puncta at the proximal end of centrioles, and this localization is dependent on CROCC and CEP250. In mitosis, when NEK2 activity increases, it localizes to centrosomes at spindle poles independent of CROCC. It further co-localizes with CDK5 in the cell-cell contacts and plasma membrane of undifferentiated and differentiated neuroblastoma cells.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
beta catenin antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to catenin beta-1 C-terminus.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Fish, Human
Shipping Conditions:
Dry Ice
Specificity:
beta catenin antibody is directed against catenin beta-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with catenin beta-1 protein from human, mouse, rat, bovine, Xenopus laevis, and Danio rerio at 100% based on homology with the immunizing sequence. Reactivity against homologues from o
Storage Conditions:
-20[o]C diluted. Avoid freeze/thaw cycles., See Manual Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of Rabbit anti-catenin B Beta-1 antibody. Lane 1: zebrafish embryos injected with myc tagged catenin B Beta 1 mRNA. Lane 2: zebrafish embryos injected with myc tagged catenin B Beta 2 mRNA. Lane 3: zebrafish embryos un-injected. Primary antibody: catenin B Beta-1 antibody at 1:500 overnight at 4°C. Secondary antibody: goat anti-rabbit HRP at 1:10,000 for 1 hour at RT. Predicted/Observed size: 85.5kDa/ ~125kDa (arrow) endogenous catenin B Beta-1. Other band(s): ~110kDa (star) co-migrating catenin B Beta-1 and B Beta-2.
Western Blot of Anti-Beta Catenin (RABBIT) Antibody. Lane 1: protein molecular weight markers. Lane 2: SK-BR-3 negative control lysate. Lane 3: MCF-7 positive control lysate. Primary Antibody: Anti-Beta Catenin at 1:1,000 for 2hr RT. Secondary Antibody: IRDye® 800CW Goat anti-Mouse Secondary Antibody (Red) and IRDye 680LT Goat Anti-Rabbit Secondary Antibody (Green) at 1:10,000 at room temperature for 45 minutes. Beta Catenin (Green) and loading control alpha-tubulin (Red). A strong specific band was observed in the positive control at the expected size (~85.5 kDa) that is not observed in the negative control. Independently Validated by antibodies-online GmbH (p/n ABIN1043907/ ABIN1043906) courtesy of ADS Biosystems Inc.
Immunohistochemistry of Rabbit anti-beta Catenin antibody. Tissue: human small intestine epithelium. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: beta Catenin antibody at 5-10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT. Localization: Strong membranous staining in a variety of epithelial tissues. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
Western Blot of Rabbit anti-Beta Catenin antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: HEK293 lysate (p/n W09-000-365). Lane 2: HeLa Lysate (p/n W09-000-363). Lane 3: MCF-7 Lysate (p/n W09-000-360). Lane 4: Jurkat Lysate (p/n W09-000-370). Lane 5: A431 Lysate (p/n W09-000-361). Lane 6: A549 Lysate (p/n W09-001-372). Lane 7: LNCap Lysate (p/n W09-001-GJ9). Lane 8: MOLT-4 Lysate (p/n W09-001-GK2). Lane 9: Ramos Lysate (p/n W09-000-GK4). Lane 10: Raji Lsyate (p/n W09-001-368). Lane 11: A-172 Lysate (p/n W09-001-GL5). Lane 12: NIH/3T3 Lysate (p/n W10-000-358). Load: 10 ug per lane. Primary antibody: Beta catenin antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 85 kDa for Beta Catenin.