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Antibodies

HMGB1 Antibody

Product Sizes
100 ug
£608.00
600-401-BS2-100UG
About this Product
SKU:
600-401-BS2
Additional Names:
HMGB1|HMGB1 Antibody, HMG1, HMG3, SBP-1, HMG1, High mobility group protein B1, High mobility group protein 1, HMG-1
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
The high-mobility group box 1 (HMGB1) protein was originally identified as a highly conserved nuclear DNA-binding protein that participates in DNA replication, repair and transcriptional regulation of gene expression (1,2). However, HMGB1 has more recently emerged as an extracellularly released mediator of inflammation and repair responses in lipopolysaccharide (LPS)-induced endotoxemia and sepsis (3). HMGB1 has also been suggested to induce secretion of pro-inflammatory cytokines and chemokines by inducing NF-kB signaling downstream of Toll-like receptor (TLR) 2 and TLR4 activation (4).
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-HMGB1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 19 amino acid peptide near the internal region of human HMGB1.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
Anti-HMGB1 antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. HMGB1 antibody is human, mouse and rat reactive.
Storage Conditions:
2-8[o]C
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunofluorescence of HMGB1. Cell: HeLa cells. Fixation: PFA-fixed. Primary Antibodies: HMGB1 at 20 ug/mL followed by goat anti-rabbit IgG antibody at 1:1000 dilution (red) and Hoechst staining (blue); alpha tubulin stained with anti-alpha tubulin antibody following by goat anti-mouse IgG antibody (green). Images were captured with confocal microscopy.
Immunohistochemistry of HMGB1. Tissue: Human Kidney Tissue. Fixation: paraffin-embedded, fixed with formaldehyde and blocked with 10% serum for 1 h at RT. Antigen retrieval: heat mediation with a citrate buffer (pH6). Primary Antibody: anti-HMGB1 antibody at 2 ug/ml overnight at 4°C. Secondary: goat anti-rabbit IgG H&L (HRP) at 1:250 was used as secondary. Counter stained with Hematoxylin.
Western Blot of HMGB1. Load: 15ug of rat tissue lysates. Primary Antibody: HMGB1 at 1ug/mL for 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry of HMGB1. Tissue: Mouse Kidney Tissue. Fixation: paraffin-embedded, fixed with formaldehyde and blocked with 10% serum for 1 h at RT. Antigen retrieval: heat mediation with a citrate buffer (pH6). Primary Antibody: anti-HMGB1 antibody at 2 ug/ml overnight at 4°C. Secondary: goat anti-rabbit IgG H&L (HRP) at 1:250 was used as secondary. Counter stained with Hematoxylin.
Western Blot of HMGB1. Load: 15ug of mouse tissue lysates. Primary Antibody: HMGB1 at 1ug/mL for 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.