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Antibodies

CRISP2 Antibody

Product Sizes
100 ug
£683.00
600-401-AN5-100UG
About this Product
SKU:
600-401-AN5
Additional Names:
CRISP2|CRISP2 Antibody, CT36, TPX1, TSP1, GAPDL5, CRISP-2, Cysteine-rich secretory protein 2, Testis-specific protein TPX-1
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
The cysteine-rich secretory proteins (CRISP) family is a group of four proteins that are strongly expressed in the male reproductive tract and have been implicated in having roles in male fertility. CRISP2, also known as TPX1, has been implicated in the adhesion between spermatids and Sertoli cells, and with CRISP1, is thought to be involved in sperm-egg fusion. CRISP2 has been shown to regulate the Ca2+ influx through ryanodine receptors (RYR) and may influence the acrosome reaction or sperm motility. CRISP2 has also been shown to bind to the mitogen-activated protein kinase kinase kinase 11 (MAP3K11) and localizes to the developing acrosome, suggesting this CRISP2-MAP3K11 complex may have a role in acrosome development.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-CRISP2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid synthetic peptide near the N-terminus of human CRISP2.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
Anti-CRISP2 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with CRISP2 from other sources has not been determined.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunofluorescence Microscopy of CRISP2 antibody. Tissue: Human testis issue. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: CRISP2 antibody at 20 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: CRISP2 is secreted into the extracellular space. Staining: CRISP2 as red fluorescent signal.
Western Blot of CRISP2 antibody. Lane 1: Human testis tissue lysate with CRISP2 antibody at 0.5 ug/mL. Lane 2: Human testis tissue lysate with CRISP2 antibody at 1 ug/mL. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 27 kDa, 27 kDa for CRISP2. Other band(s): CRISP2 splice variants and isoforms.
Immunohistochemistry of CRISP2 antibody. Tissue: Human testis tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: CRISP2 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: CRISP2 is secreted into the extracellular space. Staining: CRISP2 as precipitated brown signal with blue nuclear counterstain.