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Antibodies

CHORDC1 Antibody

Product Sizes
100 ug
600-401-AK1-100UG
About this Product
SKU:
600-401-AK1
Additional Names:
CHORDC1|CHORDC1 Antibody, CHP1, CHP1, Cysteine and histidine-rich domain-containing protein 1, CHORD domain-containing protein 1, CHORD-containing protein 1
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
The cysteine and histidine-rich domain (CHORD)-containing protein (CHORDC1) is a member of a highly conserved protein family that contains the plant protein RAR1 and the mammalian protein melusin. In mammals, CHORDC1 is an ADP-dependent HSP90-interacting protein, and this interaction is dependent on the ability of HSP90 to bind nucleotides. Recent experiments indicate that CHORDC1 mRNA is diurnally regulated in mouse hypothalamus, and that this regulation alters during development, suggesting that CHORDC1 may play a role in circadian mechanisms in the mammalian brain.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-CHORDC1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 21 amino acid synthetic peptide near the N-terminus of human CHORDC1.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Dry Ice
Specificity:
Anti-CHORDC1 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with CHORDC1 from other sources has not been determined.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of CHORDC1 antibody. Lane 1: 293 cell lysate with CHORDC1 antibody at 1 ug/mL. Lane 2: 293 cell lysate with CHORDC1 antibody at 2 ug/mL. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 37 kDa, 38 kDa for CHORDC1. Other band(s): CHORDC1 splice variants and isoforms.
Immunocytochemistry of CHORDC1 antibody. Tissue: 293 cells. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: CHORDC1 antibody at 2.5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: CHORDC1 as precipitated pink signal with blue nuclear counterstain.
Immunofluorescence Microscopy of CHORDC1 antibody. Tissue: 3T3 cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: CHORDC1 antibody at 20 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: CHORDC1 as red fluorescent signal.