Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Caspase-9 Antibody

Product Sizes
100 ug
£683.00
600-401-AE4-100UG
About this Product
SKU:
600-401-AE4
Additional Names:
CASP9|Caspase-9 Antibody, MCH6, APAF3, APAF-3, PPP1R56, ICE-LAP6, MCH6, Caspase-9, Apoptotic protease Mch-6, CASP-9
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFa, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-Caspase-9 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 20 amino acid peptide near the internal region of human Caspase-9.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
Anti-Caspase-9 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. It has no cross response to other members in caspase family.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of Caspase-9 antibody. Lane 1: HeLa whole cell lysate.. Load: 35 ug per lane. Primary antibody: Caspase-9 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 46.3 kDa, 46 kDa for Caspase-9. Other band(s): Caspase-9 splice variants and isoforms.
Immunofluorescence Microscopy of Caspase-9 antibody. Tissue: K562 cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Caspase-9 antibody at 20 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Caspase-9 is nuclear, mitochondrial, cytoplasmic, and apoptosomal. Staining: Caspase-9 as green fluorescent signal.
Immunocytochemistry of Caspase-9 antibody. Cells: K562. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Caspase-9 antibody at 2 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Caspase-9 is nuclear, mitochondrial, cytoplasmic, and apoptosomal. Staining: Caspase-9 as precipitated brown signal with hematoxylin blue nuclear counterstain.