Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Caspase-5 Antibody

Product Sizes
100 ug
600-401-AD6-100UG
About this Product
SKU:
600-401-AD6
Additional Names:
CASP5|Caspase-5 Antibody, ICH-3, ICEREL-III, ICE(rel)III, ICH3, Caspase-5, Protease ICH-3, CASP-5
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1b and IL-18. Caspase-5 can interact with caspase-1; both are constituents of the NALP1 inflammasome, a complex that can trigger the cleavage of pro-IL-1b. Expression of caspase-5 can be regulated by lipopolysaccharide (LPS) and IFN-gamma.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-Caspase-5 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 14 amino acid synthetic peptide from the internal of human Caspase-5.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
Anti-Caspase-5 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Depending on cell lines or tissues used, other cleavage products may be observed.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunocytochemistry of Caspase-5 antibody. Cell Type: P815 cells. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Caspase-5 antibody at 2 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Caspase-5 is located in the cytoplasm, neuronal cell body, neuron projection, and NLRP1 inflammasome complex. Staining: Caspase-5 is stained brown with hematoxylin purple nuclear counterstain.
Immunofluorescence Microscopy of Caspase-5 antibody. Cell Type: P815 cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Caspase-5 antibody at 2 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Caspase-5 is located in the cytoplasm, neuronal cell body, neuron projection, and NLRP1 inflammasome complex. Staining: Caspase-5 as red fluorescent signal.
Western Blot of Caspase-5 antibody in Ramos cells. Lane A: Caspase-5 antibody at 0.5 ug/mL. Lane B: Caspase-5 antibody at 1 ug/mL. Lane C: Caspase-5 antibody at 2 ug/mL. Load: 35 ug per lane. Primary antibody: Caspase-5 antibody at designated concentrations for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 128 kDa, 128 kDa for Caspase-5. Other band(s): Caspase-5 splice variants and isoforms.