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Antibodies

CARD10 Antibody

Product Sizes
100 ug
600-401-AB4-100UG
About this Product
SKU:
600-401-AB4
Additional Names:
CARD10|CARD10 Antibody, BIMP1, CARMA3, Caspase recruitment domain-containing protein 10, CARD-containing MAGUK protein 3, Carma 3
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, Bcl-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD10. This protein belongs to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARD 10 interacts with Bcl-10, a Bcl protein that promotes apoptosis, caspase-9 maturation and activation of NF-kB. CARD 10 and the related protein CARMA1 also associate with NEMO, the regulatory subunit of the IkK complex, demonstrating its importance in the regulation of NF-kB transcription factor activation.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-CARD10 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to 14 amino acids at the C-terminus of human CARD10.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Specificity:
Anti-CARD10 Antibody is immunoaffinity purified. Cross reactivity with CARD10 from other sources has not been determined.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of CARD10 antibody. Lane A: EL4 cell lysate at 5 ug/ml. Lane B: EL4 cell lysate at 10 ug/ml. Load: 35 ug per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 116 kDa, 98 kDa for CARD10. Other band(s): CARD10 splice variants and isoforms.
Immunocytochemistry of CARD10 antibody. Cell Type: EL4 cells. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: CARD10 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: CARD10 is cytoplasmic. Staining: CARD10 as brown stain with purple counterstain.
Immunofluorescence Microscopy of CARD10 antibody. Cell Type: EL4 cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: CARD10 antibody at 20 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: CARD10 is cytoplasmic. Staining: CARD10 as green fluorescent signal.
Immunohistochemistry of KLHL1 antibody. Tissue: rat brain tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: KLHL1 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: KLHL1 is cytoplasmic. Staining: KLHL1 as precipitated purple nuclear stain with hematoxylin purple nuclear counterstain.