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Antibodies

BUB3 Antibody

Product Sizes
100 ug
£683.00
600-401-AA0-100UG
About this Product
SKU:
600-401-AA0
Additional Names:
BUB3|Bub3 Antibody, BUB3L, hBUB3, Mitotic checkpoint protein BUB3
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
The mitotic checkpoint protein Bub3 is involved with the essential spindle checkpoint pathway which operates during early embryogenesis. Bub3 is important during G2 and early mitosis stages, permitting entry into mitosis depending upon the assembly state of microtubules, thus preventing premature sister chromatid separation, mis-segregation and aneuploidy. Bub3 contains four WD repeat domains and is required for the kinetochore localization of Bub1, a related kinase that is necessary for spindle assembly checkpoint function. Bub1 is able to autophosphorylate and can catalyze the phosphorylation of Bub3. Both Bub1 and Bub3 are mutually dependent for function. Altered Bub expression levels may significantly impair mitotic checkpoint function and is associated with tumor cell proliferation.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-Bub3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid synthetic peptide from near the C-terminus of human bub3.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Specificity:
Anti-Bub3 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with Bub3 from other sources has not been determined.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of Bub3 antibody. Lane A: Jurkat cell lysate at 0.5 ug/mL. Lane B: Jurkat cell lysate at 1 ug/mL. Load: 35 ug per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 37.1 kDa, ~37 kDa for Bub3.
Immunocytochemistry of Bub3 antibody. Tissue: Jurkat cells. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Bub3 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Bub3 is nuclear and occasionally cytoplasmic. Staining: Bub3 as precipitated brown signal with hematoxylin counterstain.
Immunofluorescence Microscopy of Bub3 antibody. Tissue: Jurkat cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Bub3 antibody at 20 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Bub3 as a red fluorescent signal.