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Antibodies

Beta Actin Antibody

Product Sizes
200 ug
600-401-886-200UG
About this Product
SKU:
600-401-886
Additional Names:
ACTB|rabbit anti-beta Actin Antibody, Actin Antibody, Loading Control Antibody, beta actin, B Beta actin, anti-beta actin antibody
Application:
ELISA, Immunofluorescence, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins. Anti-Actin is a loading control antibody and is critical for the correct interpretation of your western blot. Beta-Actin Loading Control Antibody is used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
beta-Actin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-Terminal region near amino acids 350-375 of Human beta Actin.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Specificity:
Anti-beta-Actin Loading Control Antibody reacts with Human, Rat, Monkey and Mouse. beta-Actin Loading Control Antibody is expected to cross-react with a wide range of species due to sequence homology. Anti-Beta Actin is affinity-purified antibody is directed against human beta Actin protein. A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of or
Storage Conditions:
2-8[o]C Aliquot. Avoid freeze/thaw cycles. Store undiluted.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of Rabbit Anti-Beta Actin Antibody. Lane 1: molecular weight. Lane 2: human embryonic kidney 293 (p/n W09-000-365). Lane 3: human lung carcinoma A549 (p/n W09-001-372). Lane 4: mouse brain (p/n W10-000-T004). Load: 35 ug per lane. Primary antibody: Beta Actin antibody at 1:1,500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO (p/n B501-0500) overnight at 4°C. Predicted/Observed size: ~42 kDa corresponding to beta Actin (arrowhead). Other band(s): none.
Immunohistochemistry of Rabbit Anti-Beta Actin Antibody. Tissue: sections 4.2 mm thick of rana pipiens tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Beta Actin antibody at 1:200 for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Beta Actin is at the neuromuscular junction. Staining: Beta Actin as precipitated green signal with red and blue counterstain.
TRPM2 levels in breast cell lines and knockdown of TRPM2 levels by RNAi silencing. (A) Immunoblot (a) and densitometric quantification (b) of TRPM2 protein levels in two noncancerous breast cell lines (HMEC, MCF-10A) and two cancerous breast cell lines (MCF-7, MDA-MB-231). Immunoblot detection of TRPM2 protein levels in human embryonic kidney cells (HEK 293) provided the positive control. (B) RNAi silencing of TRPM2 in noncancerous HMEC cells (a), and quantification of protein levels by densitometry (b). (C and D) RNAi silencing of TRPM2 in MCF-7 and MDA-MB-231 breast adenocarcinoma cells (a) and quantification of protein levels by densitometry (b). All densitometric values represent TRPM2:actin ratios. Con, untreated negative control cells; Scr, treatment with scrambled negative control siRNA oligos. Figure provided by CiteAb. Source: Int J Oncol, PMID: 25760245.
Distinct functions of HOXD proteins in regulation of neuronal differentiation.(A-B) Phase contrast imaging (A) and NEFM immunofluorescent staining (B) of BE(2)-C/Tet-Off/myc-HOXD cells cultured in the presence or absence of Doxy for 7 days. Scale bars, 50 um. Shown are representative of three independent experiments with similar results. (C) Immunoblot analysis of PHOX2B and NEFM protein levels in the same cell samples described in Figure 3. Beta-actin levels are shown as loading control. Shown are representative of two independent experiments with similar results. (D) Quantification of NEFM mRNA levels in BE(2)-C/Tet-Off/myc-HOXD cells cultured in the presence or absence of Doxy for 3 days. The NEFM mRNA levels in BE(2)-C/Tet-Off/myc-HOXD cells in the presence of Doxy were designated as 1.0 (dashed line). The data were from two independent samples with each being assayed in triplicates and analyzed using two-tailed Student's t-test with the p values indicated. Error bars, SD. Figure provided by CiteAb. Source: PLoS One, PMID: 22879880.
TRPM2 levels in breast cell lines and knockdown of TRPM2 levels by RNAi silencing. (A) Immunoblot (a) and densitometric quantification (b) of TRPM2 protein levels in two noncancerous breast cell lines (HMEC, MCF-10A) and two cancerous breast cell lines (MCF-7, MDA-MB-231). Immunoblot detection of TRPM2 protein levels in human embryonic kidney cells (HEK 293) provided the positive control. (B) RNAi silencing of TRPM2 in noncancerous HMEC cells (a), and quantification of protein levels by densitometry (b). (C and D) RNAi silencing of TRPM2 in MCF-7 and MDA-MB-231 breast adenocarcinoma cells (a) and quantification of protein levels by densitometry (b). All densitometric values represent TRPM2:actin ratios. Con, untreated negative control cells; Scr, treatment with scrambled negative control siRNA oligos. Figure provided by CiteAb. Source: Int J Oncol, PMID: 25760245.