BRRN1 Antibody
Product Sizes
100 ug
600-401-682-100UG
About this Product
- SKU:
- 600-401-682
- Additional Names:
- NCAPH|rabbit anti-BRRN-1 Antibody, BRRN1, BRRN 1, Barren Drosophila Homolog of 1 antibody, Barren homolog protein 1 antibody, BRRN antibody, CAP H antibody, CAPH antibody, Chromosome associated protein H antibody, Condensin complex subunit 2 antibody, Condensin subunit 2 antibody
- Application:
- ELISA, Western Blot
- Buffer:
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
- CE/IVD:
- RUO
- Clonality:
- Polyclonal
- Concentration:
- 0.93 mg/ml
- Extra Details:
- BRNN1, also called Barren, Condensin subunit 2, Barren homolog protein 1, Chromosome-associated protein H, hCAP-H, and XCAP-H homolog, is encoded by a gene that is a member of the barr gene family. Brnn1 is the regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. The condensin complex contains the SMC2L1 and SMC4L1 heterodimer, and three non SMC subunits that probably regulate the complex: BRRN1/CAPH, CNAP1/CAPD2 and CAPG. BRRN1 has both a nuclear and cytoplasmic localization. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2 leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase. BRRN1 is widely expressed at low level in most proliferating cells. CDC2 phosphorylates BRRN1. Its phosphorylation, as well as that of CNAP1 and CAPG subunits, activates the condensin complex and is required for chromosome condensation.
- Formulation:
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Host:
- Rabbit
- Immunogen:
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 435-460 of Human BRRN-1.
- Isotype:
- IgG
- Physical State:
- Liquid
- Reactivities:
- Human
- Shipping Conditions:
- Dry Ice
- Specificity:
- This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human BRRN1 protein. However, BLAST analysis indicates 100% homology (18/18) for this protein from human, and chimpanzee. Partial cross reactivity is expected against mouse, dog, rat chicken, and from based on sequence homolog
- Storage Conditions:
- -20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Rockland Inc
- Type:
- Antibody: Polyclonal Antibody
- Manufacturer's Data Sheet:https://www.rockland.com/search/?searchString=600-401-682