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Antibodies

Ready-To-Use RFP Antibody Pre-adsorbed

Product Sizes
100 ul
600-401-379-RTU-100UL
About this Product
SKU:
600-401-379-RTU
Additional Names:
DsRed|DsRed protein, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583, sea anemone Discosoma sp. Mushroom, RFP antibody, RFP-RTU, Ready To Use Antibody, RTU Antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
0.005 mg/ml
Extra Details:
Fluorescent proteins like green fluorescent protein (GFP) and Discosoma Red Fluorescent Protein (DsRFP) are widely used in research. DsRFP is derived from the mushroom anemone Discosoma sp. Despite sharing only 19% similarity, anti-GFP antibodies typically don't recognize DsRFP and vice versa. Structurally, DsRFP is akin to GFP in its overall B Beta-can fold and chromophore-formation chemistry. However, GFP undergoes an extra step in chromophore maturation and maintains a tetrameric structure. By using site-directed mutagenesis, various DsRFP variants have been engineered, enabling the red fluorescent protein to mature as a monomer. These monomeric DsRFP variants-mRFP1, mBanana, mCherry, mHoneydew, mPlum, mOrange, mStrawberry, and mTangerine-provide a diverse range of fluorescent colors. Rockland's RFP polyclonal antibodies, raised against the entire wild-type RFP protein, are expected to recognize all these variant forms. These antibodies have been pre-absorbed to prevent potential cross-reactivity with human, mouse, and rat serum proteins, and have also been confirmed to not react with GFP protein. All Rockland Immunochemical's RFP antibodies undergo stringent affinity purification to ensure high specificity and affinity. Thorough quality control tests guarantee that the final product meets or exceeds the highest standards, ensuring optimal performance in your assays.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full-length amino acid sequence (234aa) derived from the mushroom anemone Discosoma.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Label/Epitope-tag
Shipping Conditions:
Blue Ice
Specificity:
RTU Anti-RFP was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single pr
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
ELISA results of purified Ready-To-Use Rabbit Anti-RFP Antibody Min X Hu Ms and Rt Serum Proteins tested against RFP (Red), Mouse IgG (Blue), Human IgG (Green), and Rat IgG (Purple). Each well was coated in 1.0 ug of antigen. The starting dilution of antibody was 5 ug/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parametre curve fit where the IC50 is defined as the titer of the antibody. The curve representing the RFP as the antigen is shown in red while Mouse IgG, Human IgG, and Rat IgG antigens are shown in blue, green, and purple. Assay performed using Buffer (p/n MB-060-1000), Substrate (p/n TMB-8000), and Conjugate (Goat Anti-Rabbit IgG Antibody HRP at 1:8,500).
Western Blot Results of Ready to Use Rabbit Anti-RFP Antibody. Lane 1: recombinant RFP. Load: 0.05ug. Primary Antibody: Ready to Use Rabbit Anti-RFP Antibody 1:1000 overnight at 4°C. Secondary Antibody: Donkey Anti-Goat HRP (p/n 605-703-125) at 1:40,000 for 30 min RT. Block: BlockOut Buffer (p/n MB-073).
Western Blot of Ready-to-Use Rabbit Anti-RFP Antibody. Lane 1: Opal Prestain Molecular Weight (p/n MB-210-0500). Lane 2: HeLa (p/n W09-000-364). Lane 3: HEK293 (p/n W09-000-365). Lane 4: Cho/K1 (p/n W07-000-359). Lane 5: MDA-MB-231 (p/n W09-001-GK6). Lane 6: A431 (p/n W09-000-361). Lane 7: Jurkat (p/n W09-001-370). Lane 8: NIH/3T3 (p/n W10-000-358). Lane 9: E. coli HCP (p/n 000-001-J08). Lane 10: FLAG (p/n W00-001-383). Lane 11: RFP (p/n 000-001-379). Lane 12: GFP (p/n 000-001-215). Lane 13: GST (p/n 000-001-200). Lane 14: MBP (p/n 000-001-385). Lane 15: Opal Prestain. Primary Antibody: RTU-RFP at 1uL/mL overnight at 4°C. Secondary Antibody: Goat anti-Rabbit HRP (p/n 611-103-122) at 1:70,000 for 30min at RT. Expect 27kDa seen in lane 11. Unspecific band in lane 3 caused by cross reactivity with secondary antibody.
Western Blot of Ready-to-Use Rabbit Anti-RFP Antibody. Lane 1: Opal Prestain Molecular Weight (p/n MB-210-0500). Lane 2: RFP (p/n 000-001-379). Lane 3: Human IgG (p/n 009-0102). Lane 4: Goat IgG (p/n 005-0102). Lane 5: Mouse IgG (p/n 010-0102). Primary Antibody: RTU-RFP at 1uL/mL overnight at 4°C. Secondary Antibody: Goat anti-Rabbit HRP (p/n 611-103-122) at 1:70,000 for 30min at RT. Expect 27kDa seen in lane 2.
dYPEL3 frameshift mutations reduce the synaptic contact between nociceptors and Basin-4 neurons. (A) The syb-GRASP technique was used to report the synaptic contact between nociceptors and Basin-4. The spGFP1-10 (red cylinders) and spGFP11 (blue sectors) were expressed in nociceptors and Basin-4, respectively (left). The resulting GRASP signal was visualized by anti-GRASP antibody (green), and the spGFP1-10 that is expressed in nociceptor axon terminals was used as a normalization control (magenta) (right). Scale bar: 10 um. (B) The GRASP intensity from each neuropil was normalized by spGFP1-10 intensity and presented as mean+/-s.e.m. (left), as well as in a violin plot to show distribution (right) (n=36 for wt, n=34 for dYPEL3T1-8). Mann-Whitney test. All statistical analysis was two-tailed. **P<0.01. Figure provided by CiteAb. Source: Dis Model Mech, PMID: 32461240.