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Antibodies

APC10 Antibody

Product Sizes
100 ug
£600.00
600-401-351-100UG
About this Product
SKU:
600-401-351
Additional Names:
ANAPC10|rabbit anti-APC10 antibody, APC-10, APC 10, ANAPC10, ANAPC-10, APC, Anaphase Promoting Complex, Cyclosome subunit 10, anaphase-promoting complex subunit 10
Application:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1.5 mg/ml
Extra Details:
APC (Anaphase-Promoting Complex or Cyclosome) is a ubiquitin ligase which specifically targets mitotic regulatory factors such as Pds1/Cut2 and cyclin B. It was found that APC10/Doc1 is localized in centrosomes and mitotic spindles throughout mitosis, while it is also localized in kinetochores from prophase to anaphase and in mid-body in telophase and cytokinesis. These results strongly support the notion that human APC10/Doc1 may be one of the APC core subunits rather than the transiently associated regulatory factor.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of human APC10.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of Affinity Purified Rabbit anti-APC10. Lane 1: HeLa nuclear extract (p/n W09-001-367). Lane 2: HeLa whole cell lysate (p/n W09-000-364). Lane 3: A431 whole cell lysate (p/n W09-000-361). Lane 4: Jurkat whole cell lysate (p/n W09-001-370). Lane 5: 293 whole cell lysate (p/n W09-000-365). All lanes contain 20 ug of lysate or extract. Primary Antibody was used at a 1:500 dilution at room temperature for 1 h to detect human APC10 at ~26 kDa (predicted molecular weight is 21 kDa). After subsequent washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG (p/n 611-1302) was used for visualization. Exposure time was 4 min.
Western Blot of Affinity Purified Rabbit Anti-APC10. Lane 1: HeLa whole cell lysate (p/n W09-000-364). Lane 2: HeLa Nuclear lysate (p/n W09-001-367). Approximately 20 ug of each lysate was loaded onto a 10% SDS-PAGE gel. Primary Antibody was used at a 1:500 dilution at room temperature for 1 h. After subsequent washing, a 1:2,000 dilution of HRP conjugated Gt-a-Rabbit IgG (p/n 611-1302) was used for visualization. Exposure time was 5 min. The expected molecular weight of human APC10 is 21 kDa.