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Antibodies

Beta Amyloid Antibody

Product Sizes
25 ul
600-401-253S-25UL
About this Product
SKU:
600-401-253S
Additional Names:
APP|rabbit anti-Beta Amyloid Antibody, B Beta-amyloid, Amyloid beta A4 protein, Alzheimer disease amyloid protein, Beta amyloid, A-beta, ABPP, APPI, Beta-amyloid precursor protein
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
0.93 mg/ml
Extra Details:
Beta amyloid, often abbreviated as A-beta, is a protein that builds up in the brains of persons with Alzheimer's disease, collecting in clumps called plaques or senile plaques. While some researchers question whether beta amyloid is the cause of the dementia, most agree that it is involved in the disruption of thinking that is a hallmark of the disease. In some cases of familial Alzheimer's disease, mutations in genes for the proteins called the presenilins lead to increased production of amyloid. Researchers have been looking at how presenilin-1 in particular contributes to the excess buildup of beta amyloid. Presenilin-1 apparently acts to increase the activity of gamma-secretase, an enzyme that changes a normal protein (amyloid precursor protein or APP) into beta amyloid itself. Furthermore, presenilin-1 might be gamma-secretase.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
This antibody was affinity purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to an extracellular region of human beta amyloid conjugated to KLH using maleimide.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Specificity:
This affinity purified antibody is directed against extracellular region of beta amyloid and is useful in determining its presence in various assays. Polyclonal anti-beta amyloid detects human and mouse beta amyloid. Blast analysis of the sequence of the immunogen shows 100% identity with Human, Guinea Pig, Pig, Cyno Monkey, Dog, Polar Bear, Rabbit, Chimp, Squirrel monkey, and Sheep. Cross reactiv
Storage Conditions:
-20[o]C diluted. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunofluorescence microscopy of Rabbit Anti-Beta Amyloid antibody using HeLa cells fixed with MeOH. Anti-Beta Amyloid Antibody was used at 1 ug/mL, O/N at 4°C. Secondary antibody: Anti-RABBIT IgG DyLight™ 488 Conjugated Preadsorbed (p/n 611-741-127) at 2 ug/ml for 1 h at RT. Localization: APP is a cell surface protein that rapidly becomes internalized to endosomes and lysosomes. Some APP accumulates in secretory transport vesicles. Colocalizes with other proteins in a vesicular pattern in cytoplasm and perinuclear regions. Staining: Amyloid beta as green fluorescent signal with DAPI (blue) nuclear counterstain.
Immunohistochemistry with anti-beta amyloid antibody showing amyloid beta plaque staining in human Alzheimer's disease brain at 10x and 20x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval with E1 (Leica Microsystems) retrieval solution for 20 min and then incubated with rabbit anti-beta amyloid antibody 600-401-253 at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3'3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.
Western Blot of Rabbit Anti-Beta Amyloid Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: HEK293T Whole Cell Lysate (p/n W09-001-GX5). Lane 3: Mouse Brain Whole Cell Lysate (p/n W10-000-004). Lane 4: A-172 Whole Cell Lysate (p/n W09-001-GL5). Load: 10ug/lane. Primary Antibody: Anti-Beta Amyloid at 1ug/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated (p/n 611-103-122) at 1:70,000 for 30min at RT. Block: BlockOut Buffer (p/n MB-073). Predicted MW: ~40-50kDa. Observed MW: ~48kDa.
Western Blot of Rabbit Anti-Beta Amyloid Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: HEK293T Whole Cell Lysate (p/n W09-001-GX5). Lane 3: Mouse Brain Whole Cell Lysate (p/n W10-000-004). Lane 4: A-172 Whole Cell Lysate (p/n W09-001-GL5). Lane 5: Daudi Whole Cell Lysate (p/n W09-001-MQ2). Load: 10ug/lane. Primary Antibody: Anti-Beta Amyloid at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated (p/n 611-103-122) at 1:70,000 for 30min at RT. Block: BlockOut Buffer (p/n MB-073). Predicted MW: ~40-50kDa. Observed MW: ~48kDa.
Immunohistochemical detection of beta Amyloid using Anti-Beta Amyloid Antibody on TG APP23 mouse brain cortex frozen sections. Anti-Beta Amyloid Antibody used at 1:200 and incubated for 2 hours in TBS/BSA with Tween and azide. Fluorescent labelled anti rabbit IgG was then added. Carl Hobbs, King`s College London, United Kingdom.