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Antibodies

EEF1A2 Antibody

Product Sizes
25 ul
£254.00
600-101-ML6S-25UL
About this Product
SKU:
600-101-ML6S
Additional Names:
EEF1A2|Goat Anti-EEF1A2 Antibody, Goat Anti-Eukaryotic Translation Elongation Factor 1 Alpha 2 Antibody, Eukaryotic Elongation Factor 1 A-2, EF-1-Alpha-2, Statin-S1, EEF1AL, STN 3, Elongation Factor 1-Alpha 2, Statin-Like, EEF1A-2, EIEE33, Statin, MRD38, EF1A, STNL, HS1
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
0.77 mg/ml
Extra Details:
EEF1A2 (Eukaryotic Translation Elongation Factor 1 Alpha 2) protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. EEF1A2 encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. EEF1A2 may be associated with the development of ovarian cancer, along with Epileptic Encephalopathy, Early Infantile, 33 and Mental Retardation, Autosomal Dominant 38. Anti-EEF1A2 Antibody is useful for researchers interested in cancer research, translation factors, GTP binding, and GTPase activities.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Anti-EEF1A2 antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a near C-terminal portion of human EEF1A2 conjugated to Keyhole Limpet Hemocyanin (KLH).
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Specificity:
This affinity purified antibody is directed against human EEF1A2. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to human, mouse, rat, bovine, and rabbit.
Storage Conditions:
-20[o]C diluted. Avoid freeze/thaw cycles., See Manual Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of Goat Anti-EEF1A2 Antibody. Lane 1: Opal Pre-stained Marker (p/n MB-210-0500). Lane 2: Human Skeletal Muscle Lysate [+]. Lane 3: MCF-7 Nuclear Extract (p/n W09-001-A85) [+]. Lane 4: Mouse Adult Brain Lysate (p/n W10-000-T004) [low+]. Lane 5: Mouse Liver Lysate (p/n W10-000-T020) [-]. Load: 35ug. Primary Antibody: Anti-EEF1A2 at 1:1000 overnight at 2-8°C. Secondary Antibody: Donkey Anti-Goat HRP Antibody (p/n 605-703-125) at 1:5000 at RT for 30 min. Buffer: BlockOut buffer (p/n MB-073). Predicted: 50 kDa, Observed: ~50, 60 kDa.
Immunofluorescence of Goat Anti-EEF1A2 Antibody. Cell Line: MCF-7 cells. Fixative: 100% MeOH. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-EEF1A2 at 15ug/mL overnight at 2-8°C. Secondary Antibody: Donkey Anti-Goat DyLight™488 Antibody (p/n 605-741-125) at 5ug/mL for 1 hr at RT. Nuclear Counterstain: DAPI. Staining: (A) DAPI. (B) Anti-EEF1A2 + secondary. (C) merge A+B. (D). Secondary Only. Localization Expected: nucleoli, cytosol.
Immunohistochemistry of Goat Anti-EEF1A2 Antibody. Tissue: Human Skeletal Muscle. Fixative: none. Antigen Retrieval: HIER using Citrate buffer for 20 min. Primary Antibody: Anti-EEF1A2 at 1:100 for 30 min at RT. Secondary Antibody: Donkey Anti-Goat HRP Antibody (p/n 605-703-125) at 4uL/mL for 20 min at RT. Counterstain: Hematoxylin. Staining: EEF1A2 in human skeletal muscle shows weak to moderate cytoplasmic staining of myocytes consistent with EEF1A2.