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Antibodies

Aldh1l1 Antibody

Product Sizes
25 ug
600-101-HB6S-25UG
About this Product
SKU:
600-101-HB6S
Additional Names:
Aldh1l1|Goat Anti-Aldh1l1 Antibody, Cytosolic 10-formyltetrahydrofolate dehydrogenase, 10-FTHFDH, FDH, Aldehyde dehydrogenase family 1 member L1, Fthfd
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Aldh1l1, also known as Aldehyde Dehydrogenase 1 Family Member L1, belongs to the aldehyde dehydrogenase family. This protein catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. Loss of function or expression of this gene is associated with decreased apoptosis, increased cell motility, and cancer progression. Aldh1l1 is cytoplasmic. Diseases associated with ALDH1L1 include Methanol Poisoning and Pilocytic Astrocytoma. Anti-Aldh1l1 Antibody is useful for researchers interested in Apoptosis Research, Metabolism Research, and Cancer research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Anti-Aldh1l1 antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of mouse Aldh1l1 conjugated to Keyhole Limpet Hemocyanin (KLH).
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Mouse
Shipping Conditions:
Dry Ice
Specificity:
This affinity purified antibody is directed against mouse Aldh1l1. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to human and rat.
Storage Conditions:
-20[o]C diluted. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunofluorescence Microscopy of Goat anti-Aldh1l1 antibody. Tissue: NIH3T3. Fixation: 4% PFA. Permeabilization: 0.3%Triton X-100. Primary antibody: Aldh1l1 antibody at 15ug/mL overnight at 2-8°C. Secondary antibody: Donkey Anti-Goat IgG DyLight™ 488 Conjugated Preadsorbed (p/n 605-741-125) at 5ug/mL for 1 h at RT. Localization: cytoplasm. Staining: (A) DAPI. (B) Anti-Adlh1l1 antibody+secondary. (C) Merged A+B. (D) Secondary Only.
Immunohistochemistry of Goat Anti-Aldh1l1 antibody. Tissue: mouse Kidney tissue. Antigen retrieval: heat induced (HIER) using Citrate Buffer for 20min. Primary antibody: Aldh1l1 antibody at 1:200 for 30min at RT. Secondary Antibody: Donkey Anti-Goat HRP-IgG 4ul/mL for 20min at RT. Staining: DAB. Counter Stain: Hematoxylin. Localization: Aldh1l1 showed selective staining of renal tubular epithelial cells in mouse kidney. The specificity of the staining is cytoplasmic and membranous in tubules not in glomeruli.
Immunofluorescence Microscopy of Goat anti-Aldh1l1 antibody. Tissue: NIH3T3. Fixation: 100% MeOH. Permeabilization: 0.3%Triton X-100. Primary antibody: Aldh1l1 antibody at 15ug/mL overnight at 2-8°C. Secondary antibody: Donkey Anti-Goat IgG DyLight™ 488 Conjugated Preadsorbed (p/n 605-741-125) at 5ug/mL for 1 h at RT. Localization: cytoplasm. Staining: (A) DAPI. (B) Anti-Adlh1l1 antibody+secondary. (C) Merged A+B. (D) Secondary Only.
Immunohistochemistry of Goat Anti-Aldh1l1 antibody. Tissue: mouse Liver tissue. Antigen retrieval: heat induced (HIER) using Citrate Buffer for 20min. Primary antibody: Aldh1l1 antibody at 1:200 for 30min at RT. Secondary Antibody: Donkey Anti-Goat HRP-IgG 4ul/mL for 20min at RT. Staining: DAB. Counter Stain: Hematoxylin. Localization: Aldh1I1 showed selective cytoplasmic staining in mouse liver of hepatocytes. The specificity of the staining is weak in bile duct cells and strong membranous and cytoplasmic in hepatocytes.
Immunofluorescence Microscopy of Goat anti-Aldh1l1 antibody. Cells: HepG2 cells. Fixation: 100% MeOH. Permeabilization: 0.3%Triton X-100. Primary antibody: Anti-Aldh1l1 antibody at 15ug/mL overnight at 2-8°C. Secondary antibody: Donkey Anti-Goat IgG DyLight™ 488 Conjugated Preadsorbed (p/n 605-741-125) at 5ug/mL for 1 h at RT. Localization: cytoplasm. Images were deconvoluted. Staining: (A) DAPI. (B) Anti-Adlh1l1 antibody+secondary. (C) Merged A+B. (D) Secondary Only.