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Antibodies

PDL1 Antibody

Product Sizes
25 ul
£ POA
600-101-GU8S-25UL
About this Product
SKU:
600-101-GU8S
Additional Names:
CD274|Goat anti-PDL1 antibody, Programmed cell death 1 ligand 1, CD274, B7H1, PDCD1L1, PDCD1LG1, PDL1, B7 homolog 1, PD-L1, PDCD1 ligand 1, B7-H1, B7 homolog 1, PDL-1, CD274 molecule antibody, MGC142294 antibody, PDL 1
Application:
ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
PDL1 belongs to the immunoglobulin superfamily and BTN/MOG family. PDL1 is involved in the costimulatory signal, essential for T-cell proliferation and production of IL-10 and IFNG, in an IL-2 dependent and a PDCD1 independent manner. It is up-regulated in T-and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation. PDL1 is up-regulated in B-cells activated by surface Ig cross-linking. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Anti-PDL-1 Antibody is useful for researches interested in cancer research, NF-kB pathway research, and the Immune system.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Anti-PDL1 antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human PDL1 conjugated to Keyhole Limpet Hemocyanin (KLH).
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Specificity:
This affinity purified antibody is directed against human PDL1. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with human.
Storage Conditions:
-20[o]C diluted. Avoid freeze/thaw cycles., See Manual Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunofluorescence Microscopy of Goat anti-PDL1 antibody. Cell line: MDA-MB-231 (p/n W09-001-GK6). Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: PLD1 antibody at 10 ug/mL for overnight at 4°C. Secondary antibody: Anti-Goat IgG DyLight™488 (Rabbit) Conjugated Preadsorbed (p/n 605-441-013) at 5 ug/ml for 2 hrs at RT. Localization: PDL1 is located in the cell membrane and cytoplasm. Staining: PDL1 as green fluorescent signal with DAPI (blue) nuclear counterstain.
Western Blot of Goat anti-PDL1 antibody. Lane 1: Molecular Weight. Lane 2: r PDL1 Recombinant protein. Load: 2ug per lane. Primary antibody: PDL1 antibody at 5 ug/mL for overnight at 4°C. Secondary antibody: Dky-a-Gt IgG HRP (p/n 605-703-125) and Rb-a-Gt IgG DL488 (p/n 605-441-013) at 1:40,000 for 45 min at RT. Block: 5% w/v BSA, 1X TBS, 0.1% Tween® 20 overnight at 4°C. Predicted/Observed size: ~33 kda for PDL1. Other band(s): none.
Western Blot of Goat anti-PDL-1 antibody. Lane 1: MW ladder opal pre-stained (p/n MB-210-0500). Lane 2: U-251 WCL (p/n W09-001-GY4). Lane 3: MDA-MB-231 WCL (p/n W09-001-GK6). Lane 4: Ms Heart lysate (p/n W10-000-T014). Lane 5: recombinant human PDL-1 protein. Lane 6: A549 WCL (p/n W09-001-372). Lane 7: A549 IFNy Stimulated WCL (p/n W09-001-GY5). Load: Lysates 10ug per lane, protein at 5.0ng per lane. Primary antibody: PDL-1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: goat secondary HRP antibody (p/n 611-103-122) at 1:40,000 for 45 min at RT. Block: BlockOut (p/n MB-073) overnight at 4°C. Predicted/Observed size: 33 kDa for PDL-1.
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